Effect of scrotal heating on testicular enzymes and spermatogenesis in the rat.
Effect of scrotal heating on testicular enzymes and spermatogenesis in the rat.
复制标题
阴囊加热对大鼠睾丸酶和精子发生的影响。
DOI：
10.1071/bi9731395
复制
发表时间：
1973
期刊：
影响因子：
--
通讯作者：
P. F. Massey
中科院分区：
文献类型：
--
作者：
A. Blackshaw;D. Hamilton;P. F. Massey
将大鼠阴囊睾丸浸入42°C的水中30分钟，导致生精上皮第1、2、3期粗线期初级精母细胞迅速受损，偶尔也会造成第8期粗线期初级精母细胞受损。有细胞质嗜色与曙红或阿辛蓝和结合的荧光染料2-对甲苯胺基萘-6-磺酸的细胞膜。受损细胞迅速溶解，加热后28小时大部分细胞消失。早期圆形精子细胞也出现核变化，出现空泡化和“印戒”核。生殖细胞损伤的定量估计表明，前减数分裂阶段是最容易受到热，后期主要表现为成熟损耗的变化。酸性磷酸酶和亮氨酸氨肽酶显示早期组织化学增加有关受损粗线期初级精母细胞，这些持续到受损细胞已被删除。加热后，乳酸和琥珀酸脱氢酶的分布也发生了变化。
Immersion of the scrotal testis of the rat in water at 42°C for 30 min caused rapid damage to pachytene primary spermatocytes in stages 1, 2, 3, and occasionally 8 of the seminiferous epithelium. There was cytoplasmic chromophilia with eosin or Alcian blue and binding of the fluorescent dye 2-p-toluidinylnapthalene-6-sulphonate to the cell membranes. Rapid dissolution of damaged cells occurred and most had disappeared by 28 hr after heating. Early round spermatids also showed nuclear changes with vacuolation and the development of “signet ring” nuclei. Quantitative estimates of germ cell damage showed that the pre-meiotic stages were most susceptible to heat and that the later stages showed mainly maturation depletion changes. Acid phosphatase and leucine aminopeptidase showed early histochemical increases about the damaged pachytene primary spermatocytes and these persisted until the damaged cells had been removed. Changes in the distribution of lactate and succinate dehydrogenases were also seen after heating.