Bioavailability in humans of the flavanones hesperidin and narirutin after the ingestion of two doses of orange juice
Bioavailability in humans of the flavanones hesperidin and narirutin after the ingestion of two doses of orange juice
复制标题
DOI：
10.1038/sj.ejcn.1601547
复制
发表时间：
2003-02-01
影响因子：
4.7
通讯作者：
Rémésy, C
中科院分区：
文献类型：
--
作者：
Manach, C;Morand, C;Rémésy, C
目的：黄酮是柑橘类水果的多酚特异性，它们在高量存在。虽然柑橘类水果和果汁在世界上被广泛消费，但关于黄酮在人体中的生物利用度的信息很少。本研究的目的是确定循环代谢产物的性质，血浆动力学和尿排泄模式的黄酮，橙皮苷和纳瑞芦丁。设计：在禁食一夜之后，5名健康志愿者摄入0.5或1升商业橙汁，其中橙皮苷含量为444毫克/升，紫丁香苷含量为96.4毫克/升，同时食用不含多酚的早餐。在24小时内的10个不同时间点采集血样。收集尿液48小时，分为五组。结果：黄酮类代谢产物在摄入果汁后3 h出现在血浆中，在5 ~ 7 h达到峰值，24 h恢复到基线水平。橙皮素在摄入果汁0.5和1l后的血药浓度峰值分别为0.46 +/- 0.07 mumol/l和1.28 +/- 0.13 mumol/l。柚皮素在1l剂量后较低：0.20 +/- 0.04 μ mol/l。橙皮素的循环形式为葡萄糖醛酸酯（87%）和亚砜葡萄糖醛酸酯（13%）。对于这两种黄酮，在摄入橙汁24小时后尿液排泄几乎完全。橙皮素和柚皮素的相对尿排泄量相似，且与剂量无关：其值为摄入量的4.1 +/- 1.2至7.9 +/- 1.7%。结论：在适量或大量饮用橙汁的情况下，黄酮可能是血浆中总多酚的重要组成部分。
Objective: Flavanones are polyphenols specific of citrus fruits, where they are present in high amounts. Although citrus fruits and juices are widely consumed in the world, little information has been published on flavanone bioavailability in humans. The aim of the present study was to determine the nature of the circulating metabolites, the plasma kinetics and the urinary excretion patterns of the flavanones, hesperidin and narirutin.Design: After an overnight fast, five healthy volunteers ingested 0.5 or 1 l of a commercial orange juice providing 444 mg/l hesperidin and 96.4 mg/l narirutin, together with a polyphenol-free breakfast. Blood was sampled at 10 different timepoints over a 24 h period. Urine was collected for 48 h, in five fractions.Results: Flavanones metabolites appeared in plasma 3 h after the juice ingestion, reached a peak between 5 and 7 h, then returned to baseline at 24 h. The peak plasma concentration of hesperetin was 0.46 +/- 0.07 mumol/l and 1.28 +/- 0.13 mumol/l after the 0.5 and 1 l intake, respectively. It was lower for naringenin: 0.20 +/- 0.04 mumol/l after the 1 l dose. The circulating forms of hesperetin were glucuronides (87%) and sulphoglucuronides (13%). For both flavanones, the urinary excretion was nearly complete 24 h after the orange juice ingestion. The relative urinary excretion was similar for hesperetin and naringenin and did not depend on the dose: values ranged from 4.1 +/- 1.2 to 7.9 +/- 1.7% of the intake.Conclusions: In case of a moderate or high consumption of orange juice, flavanones may represent an important part of the pool of total polyphenols present in plasma.