Middle cerebral artery occlusion in rats: a neurological and pathological evaluation of a reproducible model.
Middle cerebral artery occlusion in rats: a neurological and pathological evaluation of a reproducible model.
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DOI：
10.1097/00006123-199207000-00014
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发表时间：
1992-07
期刊：
影响因子：
4.8
通讯作者：
St. A. Menzies;Julian T. Hoff;A. Lorris Betz
中科院分区：
文献类型：
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作者：
St. A. Menzies;Julian T. Hoff;A. Lorris Betz
大鼠大脑中动脉闭塞（MCAO）可导致不同大小的梗死。我们研究了可能影响这种变异性的三个因素：动物体重、血管解剖结构和MCAO大鼠的闭塞程度。我们还开发了一个四点神经学评估量表，并通过与四级病理学确定的梗死面积进行比较来验证其有效性。在接受标准MCAO的82只动物中，34只发生了小的皮质梗死（病理学分级I-II;梗死面积小于25 mm 2，占同侧皮质表面积的6-17%），48只发生了大的梗死（病理学分级III-IV，梗死面积大于25 mm 2，占表面积的20-56%）。在83%的动物中，我们能够从神经学评价中预测梗死的大小，并且当将I级和II级以及III级和IV级一起考虑时，该准确性达到91%（P <0.001）。在41只动物中，更广泛的血管闭塞，89%表现出大梗死。确定了四种血管模式，但没有一种在皮质卒中的发生率或大小中起重要作用。然而，体重小于300 g的大鼠显示出比大于300 g的大鼠更小的病变尺寸。我们提出的新的MCAO技术似乎是有用的再现大面积梗死的额顶叶皮层。
Middle cerebral artery occlusion (MCAO) in rats produces an infarct of varying size. We examined three factors that may influence this variability: animal weight, vascular anatomy, and extent of occlusion in rats undergoing MCAO. We also developed a four-point neurological evaluation scale and validated its usefulness by comparing it with a four-grade pathological determination of the size of the infarct. Of 82 animals subjected to a standard MCAO, 34 developed small cortical infarcts (pathological grades I-II; infarct size less than 25 mm2, 6-17% of the ipsilateral cortex surface area), and 48 large infarcts (pathological grades III-IV, infarct size greater than 25 mm2, 20-56% of surface area). We were able to predict the size of infarction from the neurological evaluation in 83% of the animals, and this accuracy reached 91% when grades I and II and III and IV were considered together (P less than 0.001). In 41 animals subjected to a more extensive vascular occlusion, 89% exhibited large infarcts. Four vascular patterns were identified but none played a significant role in the incidence or size of the cortical stroke. However, rats weighing less than 300 g showed a smaller lesion size than did rats greater than 300 g. Our proposed new MCAO technique appears useful in reproducing large-sized infarcts of the frontoparietal cortex.