Absence of estrogen receptor-α expression in human ovarian clear cell adenocarcinoma compared with ovarian serous, endometrioid, and mucinous adenocarcinoma
Absence of estrogen receptor-α expression in human ovarian clear cell adenocarcinoma compared with ovarian serous, endometrioid, and mucinous adenocarcinoma
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DOI：
10.1097/00000478-200105000-00016
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发表时间：
2001-05-01
影响因子：
5.6
通讯作者：
Saito, S
中科院分区：
文献类型：
--
作者：
Fujimura, M;Hidaka, T;Saito, S
调节卵巢透明细胞腺癌（CCA）生长的机制尚不清楚。子宫内膜异位症合并子宫内膜异位症的高发病率可能提示雌激素是子宫内膜异位症的生长促进剂。为了确定雌激素作为生长促进剂，作者研究了雌激素受体-α（ER-α）、ER-β、孕酮受体的存在或不存在。和二恶英受体（即，使用免疫组织化学方法检测临床切除的卵巢CCA、浆液性腺癌（SAC）、类卵巢腺癌（EAC）和粘液腺癌（MAC）标本中的芳香烃受体）。用逆转录-聚合酶链反应检测了三种已建立的CCA细胞系：KK，RMG-1中ER-α和ER-β信使核糖核酸的表达。MAC-II手术切除的CCA和CCA细胞系均未显示ER-α阳性染色。相反，97.2%的SAC。100%的EAC和70%的MAC显示ER-α阳性核染色（p
The mechanism that regulates growth in ovarian clear cell adenocarcinoma (CCA) is not well understood. A high incidence of concurrent endometriosis with CCA may indicate that estrogen is a growth promotor in CCA. To determine estrogen as a growth promotor, the authors investigated the presence or absence of estrogen receptor-alpha (ER-alpha), ER-beta, progesterone receptor. and dioxin receptor (i.e., aromatic hydrocarbon receptor) in clinically resected ovarian CCA, serous adenocarcinoma (SAC), endometrioid adenocarcinoma (EAC), and mucinous adenocarcinoma (MAC) specimens using an immunohistochemical method. Expression of ER-alpha and ER-beta messenger ribonucleic acid was examined by reverse transcription-polymerase chain reaction in three established CCA cell lines: KK, RMG-1. and MAC-II. None of the surgically resected CCA and CCA cell lines showed positive staining for ER-alpha. Conversely, 97.2% of SACs. 100% of EACs, and 70% of MACs showed positive nuclear staining for ER-alpha (p