Multicolor quantum dot-encoded microspheres for the detection of biomolecules
Multicolor quantum dot-encoded microspheres for the detection of biomolecules
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DOI：
10.1016/j.talanta.2007.01.058
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发表时间：
2007-06-15
期刊：
影响因子：
6.1
通讯作者：
Su, Xingguang
中科院分区：
文献类型：
--
作者：
Ma, Qiang;Wang, Xinyan;Su, Xingguang
生物相容半导体量子点具有独特的光物理性质，在荧光标记方面具有有机染料和镧系元素探针无法比拟的重要优势。在这项工作中，我们通过逐层组装的方法制备了多色量子量子点编码微球。以直径为3微米的聚苯乙烯微球为模板，通过静电作用制备了不同尺寸的镉镉量子点/聚电解质多层膜。用抗人和抗兔两种不同抗体制备了两种具有生物功能的多色微球。人免疫球蛋白和兔免疫球蛋白可作为多重荧光免疫分析的靶抗原。此外，研制了一种新型的微流控芯片装置，用于检测两种抗原偶联的多色QD编码微球；根据其荧光信号可以区分这两种微球。(C)2007 Elsevier B.V.保留所有权利。
The biocompatible semiconductor quantum dots (QDs) have unique photophysical properties, which provide important advantages over organic dyes and lanthanide probes in fluorescence labeling applications. In this work, multicolor quantum QD-encoded microspheres have been prepared via the layer-by-layer (LbL) assembly approach. Polystyrene microspheres of 3 mu m diameter were used as templates for the deposition of different sized CdTe QDs/polyelectrolyte multilayers via electrostatic interactions. Two kinds of biofuntional multicolor microspheres with two different antibodies, anti-human IgG and anti-rabbit IgG were prepared. Human IgG and rabbit IgG can be detected as target antigens in the multiplexed fluoroimmunoassays. Furthermore, a novel microfluidic on-chip device was developed to detect two kinds of antigen-conjugated multicolor QD-encoded microspheres; the microspheres can be distinguished from each other based on their fluorescence signals. (C) 2007 Elsevier B.V. All rights reserved.