Chromophoric factors F342 and F430 of Methanobacterium thermoautotrophicum
Chromophoric factors F342 and F430 of Methanobacterium thermoautotrophicum
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热自养甲烷杆菌发色因子 F342 和 F430
DOI：
10.1111/j.1574-6968.1978.tb01916.x
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发表时间：
1978
影响因子：
2.1
通讯作者：
R. Wolfe
中科院分区：
文献类型：
--
作者：
R. Gunsalus;R. Wolfe
产甲烷细菌已被证明具有三种独特的辅酶，到目前为止还没有在自然界的其他地方发现。辅酶M，2-巯基乙磺酸是一种甲基转移辅酶，其甲基化衍生物2-（甲硫基）-乙磺酸作为产甲烷菌甲基还原酶系统的底物[1-3]。辅酶F420是一种低电位电子载体，在氢化酶和NADP之间或甲酸盐和NADP之间介导电子[4-6]。甲基还原酶系统最近已被解析为一种不能被任何已知辅酶取代的小的氧敏感辅因子[7]。除这些辅酶外，还在产甲烷菌（Methabacterium thermoautotrophicum）的提取物中发现了两种新的因子。我们在这里报告的性质，这些因素被指定为F342和F43 o的主要吸收带在342 nm和430 nm分别。
Methanogenic bacteria have been shown to possess three unique coenzymes which so far have not been found elsewhere in nature. Coenzyme M, 2-mercaptoethanesulfonic acid is a methyl-transfer coenzyme, its methylated derivative, 2-(methylthio)-ethanesulfonic acid serving as the substrate for the methylreductase system of methanogens [1-3]. Coenzyme F420 is a low potential electron carrier, mediating electrons between hydrogenase and NADP or between formate and NADP [4-6]. The methylreductase system recently has been resolved for a small oxygen-sensitive cofactor which cannot be replaced by any of the known coenzymes [7]. In addition to these coenzymes two new factors have been found in extracts of the methanogen, Metha-nobacterium thermoautotrophicum. We report here on the properties of these factors which are designated F342 and F43 o for their major absorption bands at 342 nm and 430 nm respectively.