Lactobacillus rhamnosus EM1107 in goat milk matrix modulates intestinal inflammation involving NF-κB p65 and SOCs-1 in an acid-induced colitis model
Lactobacillus rhamnosus EM1107 in goat milk matrix modulates intestinal inflammation involving NF-κB p65 and SOCs-1 in an acid-induced colitis model
复制标题
羊奶基质中的鼠李糖乳杆菌 EM1107 在酸诱导的结肠炎模型中调节涉及 NF-κB p65 和 SOCs-1 的肠道炎症
DOI：
10.1016/j.jff.2018.09.013
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
R. Queiroga
中科院分区：
文献类型：
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作者：
R. Rodrigues;G. Guerra;J. Soares;K. Santos;F. R. L. Rolim;Paloma Oliveira Antonino de Assis;D. F. S. Araújo;R. F. A. Júnior;V. B. Garcia;A. Araújo;R. Queiroga
炎症性肠病(IBD)的特点是免疫反应紊乱。益生菌能够调节肠道微生物区系，调节炎症反应。本研究评价了鼠李糖乳杆菌EM1107、单独(EM1107)和添加山羊奶酪(LRC)对大鼠乙酸性结肠炎的影响。EM1107或LRC对肠道炎症有保护作用，能改善疾病活动性指数，减轻炎症所致的肉眼损伤。还可降低髓过氧化物酶、肿瘤坏死因子-α、白介素1-β水平，改善氧化应激。组织学评价证实了其在结肠组织保存中的有益作用。肠组织免疫组织化学分析显示IL-17、NF-κB p65、MMP2、MMP9表达下调，肠粘膜重要屏障蛋白SOCS-1、ZO-1和MUC2表达上调。这些结果表明，单独添加鼠李糖乳杆菌EM1107或添加到山羊干酪中都能预防醋酸引起的损伤，有可能成为人IBD的替代品。
Inflammatory Bowel Diseases (IBD) is characterized by a deregulated immune response. Probiotics are able to modulate the intestinal microbiota and regulating inflammatory responses. This study evaluated the effect of pretreatment ofLactobacillus rhamnosusEM1107, isolated (EM1107) or added to goat cheese (LRC), on acetic acid-induced colitis in rats. EM1107 or LRC had a protective effect on intestinal inflammation, with the improvement of disease activity index and macroscopic damage caused by inflammation. It also reduced myeloperoxidase, TNF-α, IL-1β colonic levels and improved oxidative stress. The histological evaluation confirmed the beneficial effect in colonic tissue preservation. Intestinal immunohistochemical analysis revealed down-regulation of IL-17, NF-κB p65, MMP-2, MMP-9, and iNOS expression, up-regulation of SOCs-1, ZO-1 and MUC-2, which are important intestinal mucosal barrier proteins. These results suggest thatLactobacillus rhamnosusEM1107 alone or added to goat cheese exerts a preventive effect against acetic acid-induced damage and may be an alternative to human IBD.
DOI：
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发表时间：
2013
期刊：
--
影响因子：
--
作者：
Rana Al-Sadi;Shuhong Guo;D. Ye;K. Dokladny;T. Alhmoud;Lisa Ereifej;H. Said;T. Ma
通讯作者：
Rana Al-Sadi;Shuhong Guo;D. Ye;K. Dokladny;T. Alhmoud;Lisa Ereifej;H. Said;T. Ma