Reduced mitochondrial density in the vastus lateralis muscle of patients with COPD
Reduced mitochondrial density in the vastus lateralis muscle of patients with COPD
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DOI：
10.1183/09031936.00146906
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发表时间：
2007-07-01
影响因子：
24.3
通讯作者：
Schols, A. M. W. J.
中科院分区：
文献类型：
--
作者：
Gosker, H. R.;Hesselink, M. K. C.;Schols, A. M. W. J.
骨骼肌功能障碍是慢性阻塞性肺疾病（COPD）导致运动不耐受的公认标志。COPD患者的股外侧肌的特征在于线粒体酶活性降低;然而，胫骨前部的情况并非如此。然而，目前还不清楚是否在股动脉的氧化能力受损是由于线粒体体积density.Muscle活检获得6名COPD患者和4名健康的年龄匹配的对照组的股外侧肌，并从胫骨前的另外6名COPD患者和6名对照组。线粒体数量、面积分数和形态计量学以及Z线宽度（作为纤维类型的替代标记）用透射电镜分析，COPD患者股肌线粒体数量（0.34对0.63 n μ m（-2））和面积分数（1.95对4.25%）与对照组相比减少。尽管线粒体数量减少（0.65 vs n.mu（-2）），但COPD患者胫骨的线粒体面积分数与对照组相比保持不变。可以得出结论，线粒体面积分数减少可能导致慢性阻塞性肺疾病患者股肌氧化能力降低，而胫骨线粒体面积分数保持不变可能解释正常的氧化能力。
Skeletal muscle dysfunction is a well-recognised hallmark of chronic obstructive pulmonary disease (COPD) leading to exercise intolerance. The vastus lateralis of COPD patients is characterised by reduced mitochondrial enzyme activity; however, this is not the case in the tibialls anterior. It is, however, unclear whether the compromised oxidative capacity in the vastus is due to reduced mitochondrial volume density.Muscle biopsies were obtained from the vastus lateralis of six COPD patients and four healthy age-matched controls, and from the tibialis anterior of another six COPD patients and six controls. Mitochondrial number, fractional area and morphometry, as well as Z-line width (as a surrogate marker of fibre type), were analysed using transmission electron microscopy.Mitochondrial number (0.34 versus 0.63 n mu m(-2)) and fractional area (1.95 versus 4.25%) were reduced in the vastus of COPD patients compared with controls. Despite a reduced mitochondrial number (0.65 versus 0.88 n.mu m(-2)), the mitochondrial fractional area was maintained in the tibialis of COPD patients compared with controls.It can be concluded that the reduced mitochondrial fractional area is likely to contribute to the decreased oxidative capacity in the vastus of chronic obstructive pulmonary disease patients, whereas the maintained mitochondrial fractional area in the tibialls may explain the normal oxidative capacity.