Characterizing coinfection in children with COVID-19: A dual center retrospective analysis.

Characterizing coinfection in children with COVID-19: A dual center retrospective analysis.
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DOI:
10.1017/ice.2020.1221
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发表时间:
2021-09
影响因子:
4.5
通讯作者:
Kumar M
Kumar M
中科院分区:
医学4区
文献类型:
--
作者:
Zhang DD;Acree ME;Ridgway JP;Shah N;Hazra A;Ravichandran U;Kumar M

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MethodsWe retrospectively reviewed electronic health records of all pediatric patients tested for severe acute respiratory coronavirus virus 2 (SARS-CoV-2) from March 9, 2020, through April 30, 2020, in 2 Chicagoland medical centers. At the University of Chicago Medicine, SARS-CoV-2 was diagnosed using one of the following real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays on respiratory specimens: Cobas SARS-CoV-2 RT-PCR assay (Roche Basel, Switzerland) or Xpert Xpress SARS-CoV-2 test (Cepheid, Sunnyvale, CA). Respiratory coinfections were primarily identified using a multiplex RT-PCR respiratory viral panel (RVP) with the following targets: adenovirus, coronavirus 229E/HKU1/NL63/OC43, human metapneumovirus, influenza-A/-B, parainfluenzas 1–4, respiratory syncytial virus, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Bordetella pertussis, and rhinovirus/enterovirus (FilmArray Respiratory Panel, BioFire Diagnostics, Salt Lake City, UT). Coinfections were also identified using the influenza/respiratory syncytial virus (RSV) RT-PCR assay (Cepheid Xpert Xpress Flu/RSV) known as the influenza/RSV panel (IRP). At NorthShore University HealthSystem, SARS-CoV-2 was identified similarly using RT-PCR: Xpert Xpress or BD Max (Becton Dickinson, Franklin Lakes, NJ). Coinfections were detected using a multiplex RT-PCR panel that contained only the viral targets of the RVP (GenMark Dx, GenMark Diagnostics, Carlsbad, CA), as well as an IRP (Roche Cobas Liat Influenza A/B and RSV). We included all RVPs and IRPs that were obtained within 7 days of a SARS-CoV-2 test.
DOI: 10.1542/peds.2020-0961
发表时间: 2020-07-01
期刊: PEDIATRICS
影响因子: 8
作者:
Wu, Qin;Xing, Yuhan;Xing, Quansheng
通讯作者: Xing, Quansheng
DOI: 10.1093/jpids/piaa070
发表时间: 2020-11-01
影响因子: 3.2
作者:
Mannheim, Jonathan;Gretsch, Stephanie;Fricchione, Marielle J.
通讯作者: Fricchione, Marielle J.