MUTATIONS THAT IMPAIR A POSTTRANSCRIPTIONAL STEP IN EXPRESSION OF HLA-A AND HLA-B ANTIGENS
MUTATIONS THAT IMPAIR A POSTTRANSCRIPTIONAL STEP IN EXPRESSION OF HLA-A AND HLA-B ANTIGENS
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DOI：
10.1073/pnas.82.23.8183
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
ORR, HT
中科院分区：
文献类型：
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作者：
DEMARS, R;RUDERSDORF, R;ORR, HT
突变会干扰 HLA-A 和 -B 基因的转录后表达。对含有一份主要组织相容性复合物 (MHC) 的 B 类淋巴母细胞进行诱变和免疫选择，以获得 MHC 抗原丢失突变体。尽管 HLA-A 和 -B 基因存在并转录，但一些突变部分降低了 HLA-A 的表面表达，并同时消除了 HLA-B 表达。在这些突变体与其他 B 类淋巴母细胞的杂交体中，抗原表达完全恢复。因此，B淋巴母细胞上HLA-A和-B抗原的正常细胞表面表达需要(i)在HLA-A和-B基因转录后在抗原的产生中执行至少一个反式活性步骤，或(ii)将I类抗原与其他分子结合。一种突变体的DNA分析表明HLA-A和-B抗原正常表达所需的基因座可能位于MHC补体基因和HLA-DPαII基因座之间。
Mutations can interfere with posttranscriptional expression of the HLA-A and -B genes. B-lymphoblastoid cells that contain one copy of the major histocompatibility complex (MHC) were subjected to mutagenesis and immunoselection for MHC antigen-loss mutants. Some mutations partially reduced surface expression of HLA-A and eliminated HLA-B expression concurrently, although the HLA-A and -B genes were present and transcribed. Antigen expression was fully restored in hybrids of these mutants with other B-lymphoblastoid cells. Therefore, normal cell surface expression of the HLA-A and -B antigens on B lymphoblasts requires (i) execution of at least one trans-active step in the production of the antigens after transcription of the HLA-A and -B genes or (ii) association of the class I antigens with other molecules. DNA analysis of one mutant suggests the possibility that a locus required for the normal expression of the HLA-A and -B antigens is located between the MHC complement genes and the HLA-DP .alpha.II locus.