Macroscopic electrical propagation in the guinea pig urinary bladder
Macroscopic electrical propagation in the guinea pig urinary bladder
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DOI：
10.1152/ajprenal.00215.2014
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发表时间：
2014-07-15
影响因子：
4.2
通讯作者：
Lammers, W. J.
中科院分区：
文献类型：
--
作者：
Hammad, F. T.;Stephen, B.;Lammers, W. J.
关于膀胱壁中的宏观电传播的知识很少。同时记录大量细胞外电极是一种可用于研究宏观电传播模式的技术。将14只豚鼠的膀胱分离并置于器官浴中。将16 x 4电极阵列定位在Serpentine膀胱表面的各个部位，并在不同的膀胱内体积下进行记录。在四个实验中，将卡巴胆碱（CCH; 10（-6）M）、硝苯地平（10 mM）或河豚毒素（TTX; 10（-6）M）加入到灌注液中。实验结束后，对细胞外信号进行了分析，并构建了传播图。在膀胱表面的所有部位检测到电波，并在自发终止之前传播有限的距离。大多数波（>90%）在轴向方向上传播（即，从圆顶到底座或反之亦然）。囊泡体积的增加显著降低了传导速度（从4.9 +/- 1.5到2.7 +/- 0.7 cm/s; P < 0.05）。CCH升高，硝苯地平降低，而TTX对电活动影响不大。此外，发现了一种新的电现象，称为“补丁”，由此在膀胱表面的区域上检测到同时的电偏转。在膀胱表面上检测到两种类型的电活动：1）电波优先在轴向方向上传播和2）电斑。传播的电波可以形成充盈阶段期间膀胱中局部自发收缩的基础。
There is little knowledge about macroscopic electrical propagation in the wall of the urinary bladder. Recording simultaneously from a large number of extracellular electrodes is one technology that could be used to study the patterns of macroscopic electrical propagations. The urinary bladders from 14 guinea pigs were isolated and placed in an organ bath. A 16 x 4-electrode array was positioned at various sites on the serosal bladder surface, and recordings were performed at different intravesical volumes. In four experiments, carbachol (CCH; 10(-6) M), nifedipine (10 mM), or tetrodotoxin (TTX; 10(-6) M) was added to the superfusing fluid. After the experiments, the extracellular signals were analyzed and propagation maps were constructed. Electrical waves were detected at all sites on the bladder surface and propagated for a limited distance before terminating spontaneously. The majority of waves (>90%) propagated in the axial direction (i.e., from dome to base or vice versa). An increase in vesicle volume significantly decreased the conduction velocity (from 4.9 +/- 1.5 to 2.7 +/- 0.7 cm/s; P < 0.05). CCH increased, nifedipine decreased, while TTX had little effect on electrical activities. In addition, a new electrical phenomenon, termed a "patch," was discovered whereby a simultaneous electrical deflection was detected across an area of the bladder surface. Two types of electrical activities were detected on the bladder surface: 1) electrical waves propagating preferentially in the axial direction and 2) electrical patches. The propagating electrical waves could form the basis for local spontaneous contractions in the bladder during the filling phase.