Novel avian influenza A (H5N6) viruses isolated in migratory waterfowl before the first human case reported in China, 2014.
Novel avian influenza A (H5N6) viruses isolated in migratory waterfowl before the first human case reported in China, 2014.
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2014 年中国报告首例人类病例之前，在迁徙水禽中分离出新型甲型禽流感 (H5N6) 病毒。
DOI：
10.1038/srep29888
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发表时间：
2016-07-19
影响因子：
4.6
通讯作者：
Gao GF
中科院分区：
文献类型：
--
作者：
Bi Y;Liu H;Xiong C;Di Liu;Shi W;Li M;Liu S;Chen J;Chen G;Li Y;Yang G;Lei Y;Xiong Y;Lei F;Wang H;Chen Q;Chen J;Gao GF
2014年5月，中国正式确认四川省首例人感染新型H5N6禽流感病毒。在报告首例人感染禽流感病例之前，在对野生鸟类的禽流感监测中，从湖北省辰湖湿地候鸟的粪便样本中检测到三种H5N6病毒，中国说。基因和系统发育分析显示，这三种新病毒与2014年以来导致中国人感染的H5N6病毒关系密切。对所有八个片段的贝叶斯系统发育重建表明，在这些病毒的进化过程中发生了多次重新分类事件。血凝素(HA)和神经氨酸酶(NA)分别来自H5N2和H6N6禽流感病毒，而所有6个内部基因都来自H5N1禽流感病毒。这种重新组合可能发生在2012年至2013年间的中国东部。对HA和NA基因的系统地理分析将病毒追溯到中国南部，从那里它们通过中国东部传播到其他地区。受体结合试验表明，来自迁徙水禽的H5N6病毒具有与人型受体结合的活性，表明有可能传播给人类。这些数据表明，迁徙的水禽可能在新型H5N6病毒的传播中发挥了作用。
In May 2014, China formally confirmed the first human infection with the novel H5N6 avian influenza virus (AIV) in Sichuan Province. Before the first human case was reported, surveillance of AIVs in wild birds resulted in the detection of three H5N6 viruses in faecal samples from migratory waterfowl in Chenhu wetlands, Hubei Province, China. Genetic and phylogenetic analyses revealed that these three novel viruses were closely related to the H5N6 virus that has caused human infections in China since 2014. A Bayesian phylogenetic reconstruction of all eight segments suggests multiple reassortment events in the evolution of these viruses. The hemagglutinin (HA) and neuraminidase (NA) originated from the H5N2 and H6N6 AIVs, respectively, whereas all six internal genes were derived from avian H5N1 viruses. The reassortant may have occurred in eastern China during 2012–2013. A phylogeographic analysis of the HA and NA genes traced the viruses to southern China, from where they spread to other areas via eastern China. A receptor-binding test showed that H5N6 viruses from migratory waterfowl had human-type receptor-binding activity, suggesting a potential for transmission to humans. These data suggest that migratory waterfowl may play a role in the dissemination of novel H5N6 viruses.