Molecular cloning and functional analysis of the FLOWERING LOCUS T (FT) homolog GhFT1 from Gossypium hirsutum
Molecular cloning and functional analysis of the FLOWERING LOCUS T (FT) homolog GhFT1 from Gossypium hirsutum
复制标题
陆地棉 FLOWERING LOCUS T (FT) 同源物 GhFT1 的分子克隆和功能分析
DOI：
10.1111/jipb.12316
复制
发表时间：
2015-06-01
影响因子：
11.4
通讯作者：
Huang, Xianzhong
中科院分区：
文献类型：
--
作者：
Guo, Danli;Li, Chao;Huang, Xianzhong
FLOWERING LOCUS T (FT) 编码磷脂酰乙醇胺结合蛋白 (PEBP) 家族的成员，充当移动花信号，在调节被子植物花转变中发挥重要作用。我们从陆地棉品种中分离得到一个FT同源物（GhFT1），新陆早33号GhFT1主要在雄蕊和萼片中表达，并且在纤维发育起始阶段有相对较高的表达水平。 GhFT1 mRNA 在长日照和短日照条件下均表现出昼夜振荡，表明该基因的表达可能受到生物钟的控制。亚细胞分析显示GhFT1蛋白定位于细胞质和细胞核。与野生型植物相比，转基因拟南芥植物中 GhFT1 的异位表达导致开花提前。此外，拟南芥ft-10突变体中GhFT1的异位表达部分挽救了极晚开花表型。最后，在 35S::GhFT1 转基因拟南芥植物中，AtFT 下游的几个开花相关基因高度上调。总之，GhFT1是棉花中的FT同源基因，与其他植物物种中的直系同源基因相似，可调节花的转变，因此它可能是促进棉花育种早期成熟的候选靶标。
FLOWERING LOCUS T (FT) encodes a member of the phosphatidylethanolamine-binding protein (PEBP) family that functions as the mobile floral signal, playing an important role in regulating the floral transition in angiosperms. We isolated an FT-homolog (GhFT1) from Gossypium hirsutum L. cultivar, Xinluzao 33 GhFT1 was predominantly expressed in stamens and sepals, and had a relatively higher expression level during the initiation stage of fiber development. GhFT1 mRNA displayed diurnal oscillations in both long-day and short-day condition, suggesting that the expression of this gene may be under the control of the circadian clock. Subcellular analysis revealed that GhFT1 protein located in the cytoplasm and nucleus. Ectopic expression of GhFT1 in transgenic arabidopsis plants resulted in early flowering compared with wild-type plants. In addition, ectopic expression of GhFT1 in arabidopsis ft-10 mutants partially rescued the extremely late flowering phenotype. Finally, several flowering related genes functioning downstream of AtFT were highly upregulated in the 35S::GhFT1 transgenic arabidopsis plants. In summary, GhFT1 is an FT-homologous gene in cotton that regulates flower transition similar to its orthologs in other plant species and thus it may be a candidate target for promoting early maturation in cotton breeding.