Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose.
Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose.
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DOI：
10.1186/1477-5956-9-66
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发表时间：
2011-10-18
期刊：
影响因子：
2
通讯作者：
Lefsrud MG
中科院分区：
文献类型：
--
作者：
Sivagnanam K;Raghavan VG;Shah M;Hettich RL;Verberkmoes NC;Lefsrud MG
丁醇是丙酮丁醇梭菌通过丙酮-丁醇-乙醇（ABE）发酵工艺生产的第二代生物燃料。鸟枪蛋白质组学为深入研究生物体的整个蛋白质组提供了一种直接的方法。本文主要研究了C.利用葡萄糖和木糖从ABE发酵中获得丙酮丁醇梭菌，以了解其功能机制。参与丁醇生产的acetobutylicum蛋白。我们在C.采用二维液相色谱-串联质谱法（2D-LC-MS/MS）对ABE发酵过程中的丙酮丁醇进行了分离鉴定。这包括来自葡萄糖的717种蛋白质和来自木糖底物的826种蛋白质。共发现649个蛋白质是共同的，22个显着差异表达的蛋白质之间的葡萄糖和木糖底物。我们的研究结果表明，鞭毛蛋白是高度上调与葡萄糖相比，木糖底物在ABE发酵。还发现由于不存在CheW和CheV蛋白质，木糖底物的趋化活性丧失。这是首次对C.从单个时间数据点的葡萄糖和木糖底物之间的ABE发酵中的丙酮丁醇菌ATCC 824，并且在此鉴定的蛋白质的数量比在该生物体上进行的任何其他研究都多，直到本报告。
Butanol is a second generation biofuel produced by Clostridium acetobutylicum through acetone-butanol-ethanol (ABE) fermentation process. Shotgun proteomics provides a direct approach to study the whole proteome of an organism in depth. This paper focuses on shotgun proteomic profiling of C. acetobutylicum from ABE fermentation using glucose and xylose to understand the functional mechanisms of C. acetobutylicum proteins involved in butanol production. We identified 894 different proteins in C. acetobutylicum from ABE fermentation process by two dimensional - liquid chromatography - tandem mass spectrometry (2D-LC-MS/MS) method. This includes 717 proteins from glucose and 826 proteins from the xylose substrate. A total of 649 proteins were found to be common and 22 significantly differentially expressed proteins were identified between glucose and xylose substrates. Our results demonstrate that flagellar proteins are highly up-regulated with glucose compared to xylose substrate during ABE fermentation. Chemotactic activity was also found to be lost with the xylose substrate due to the absence of CheW and CheV proteins. This is the first report on the shotgun proteomic analysis of C. acetobutylicum ATCC 824 in ABE fermentation between glucose and xylose substrate from a single time data point and the number of proteins identified here is more than any other study performed on this organism up to this report.
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