Bioorthogonal Turn-On Probe Based on Aggregation-Induced Emission Characteristics for Cancer Cell Imaging and Ablation
Bioorthogonal Turn-On Probe Based on Aggregation-Induced Emission Characteristics for Cancer Cell Imaging and Ablation
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DOI：
10.1002/anie.201601744
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发表时间：
2016-05-23
影响因子：
16.6
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Yuan, Youyong;Xu, Shidang;Liu, Bin
生物正交开启探针已被广泛应用于各种生物过程的可视化。目前可用的大多数生物正交开启探针都是以叠氮或四氮为官能团的蓝色或绿色发光荧光团。在这里，我们提出了一种基于具有聚集诱导发射特征的红色发射荧光(AIEgen)的生物正交开启探针的设计的替代策略。该探针是水溶性和非荧光的，因为AIEgen通过自由分子运动消耗能量，但一旦与癌细胞表面叠氮功能化的多糖发生点击反应，荧光就会立即打开。荧光的开启归因于AIEgen分子运动的限制，AIEgen填充了辐射衰变通道。此外，AIEgen在可见光(波长=400-700 nm)照射下可以产生活性氧物种(ROS)，显示了其作为成像和光疗剂的双重作用。
Bioorthogonal turn-on probes have been widely utilized in visualizing various biological processes. Most of the currently available bioorthogonal turn-on probes are blue or green emissive fluorophores with azide or tetrazine as functional groups. Herein, we present an alternative strategy of designing bioorthogonal turn-on probes based on red-emissive fluorogens with aggregation-induced emission characteristics (AIEgens). The probe is water soluble and non-fluorescent due to the dissipation of energy through free molecular motion of the AIEgen, but the fluorescence is immediately turned on upon click reaction with azide-functionalized glycans on cancer cell surface. The fluorescence turn-on is ascribed to the restriction of molecular motion of AIEgen, which populates the radiative decay channel. Moreover, the AIEgen can generate reactive oxygen species (ROS) upon visible light (lambda = 400-700 nm) irradiation, demonstrating its dual role as an imaging and phototherapeutic agent.