DEVELOPMENTAL-CHANGES IN LAYER I OF THE HUMAN NEOCORTEX DURING PRENATAL LIFE - A DII-TRACING AND ACHE AND NADPH-D HISTOCHEMISTRY STUDY
DEVELOPMENTAL-CHANGES IN LAYER I OF THE HUMAN NEOCORTEX DURING PRENATAL LIFE - A DII-TRACING AND ACHE AND NADPH-D HISTOCHEMISTRY STUDY
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DOI：
10.1002/cne.903380302
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发表时间：
1993-12-15
影响因子：
2.5
通讯作者：
GONZALEZHERNANDEZ, T
中科院分区：
文献类型：
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作者：
MEYER, G;GONZALEZHERNANDEZ, T
通过使用 Dil 示踪、AChE 和 NADPH-d 组织化学，并检查 Yakovlev Collection 的尼氏染色材料，研究了人类新皮质中胎儿 I 层（边缘区，MZ）的发育。我们描述了 Cajal-Retzius (CR) 细胞和软膜下颗粒层 (SGL) 颗粒细胞的顺序成熟，以及两者之间的密切关系。第一个 CR 细胞在妊娠 6 周 (GW) 时出现在原始丛状层中。皮质板形成后，它们定居在软脑膜表面下方。 13 GW 时，SGL 开始在 CR 电池周围形成。 CR细胞多态性群体的水平成员在13GW时开始成熟，中间形式和垂直形式分别在16GW和18GW时分化。所有 CR 细胞都投射到 MZ 下三分之一的轴突丛中。从 18 GW 开始，CR 细胞和 SGL 开始分离，多态性 CR 细胞现在位于 SGL 下方，它们通过上升过程与 SGL 保持连接。颗粒细胞侵入下MZ接触CR细胞的体细胞和突起。垂直 CR 细胞的体细胞伸长至 23/24 GW，此时出现退化迹象。 24 GW 后，所有多态性 CR 细胞均死亡。颗粒细胞在24GW后退化； SGL 在 28/30 GW 时消失。一群持续存在的 CR 细胞在形态上与短暂的多态性不同，出现在软膜下位置，并在整个生命过程中少量存活。小的非 CR 神经元首先在 I 层的下半部分分化，然后也在上半部分分化。组织化学上，所有 CR 细胞均为 AChE 阳性；它们仅在 20 GW 时短暂含有 NADPH-d。我们认为，当分子层的确定纤维系统尚未建立时，CR 细胞和 SGL 为发育中的皮质板提供瞬时神经支配网络。 (C) 1993 Wiley-Liss, Inc.
The development of fetal layer I (marginal zone, MZ) was studied in the human neocortex by using Dil tracing and AChE and NADPH-d histochemistry, and examining the Nissl-stained material of the Yakovlev Collection. We describe the sequential maturation of the Cajal-Retzius (CR) cells and the granule cells of the subpial granular layer (SGL), and the close relationship between both. The first CR cells appear in the primordial plexiform layer, at 6 gestational weeks (GW). After the formation of the cortical plate, they settle under the pial surface. At 13 GW, the SGL begins to form around the CR cells. The horizontal members of a polymorphic population of CR cells begin to mature at 13 GW, and the intermediate and vertical forms differentiate at 16 and 18 GW, respectively. All CR cells project into an axonal plexus in the lower third of the MZ. From 18 GW, CR cells and SGL become segregated, and the polymorphic CR cells lie now below the SGL, with which they remain connected by ascending processes. Granule cells invade the lower MZ contacting somata and processes of CR cells. The somata of vertical CR cells elongate until 23/24 GW when they show degenerative signs. After 24 GW, all polymorphic CR cells die. Granule cells degenerate after 24 GW; the SGL disappears at 28/30 GW. A population of persisting CR cells, morphologically different from the transient polymorphic forms, appears in a subpial position and survives in small numbers throughout life. Small non-CR neurons differentiate first in the lower half of layer I, thereafter also in the upper half. Histochemically, all CR cells are AChE-positive; they contain NADPH-d only transiently at 20 GW. We propose that CR cells and SGL provide a transient innervation network for the developing cortical plate at a time when the definitive fiber systems of the molecular layer are not yet established. (C) 1993 Wiley-Liss, Inc.