DNA molecular markers of Mytilopsis sallei in three bays,for genetic difference
DNA molecular markers of Mytilopsis sallei in three bays,for genetic difference
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发表时间：
2006
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通讯作者：
Zhou Xi-ping;Yang Li;Cai Li-zhe;Lin Xiu-chun;Liu Wei-ming;Jin Liang;Lin Rong-cheng
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作者：
Zhou Xi-ping;Yang Li;Cai Li-zhe;Lin Xiu-chun;Liu Wei-ming;Jin Liang;Lin Rong-cheng
采用RAPD（随机扩增多态性DNA）技术对三个海湾的沙氏贻贝3个居群进行遗传差异分析。结果表明，东山湾和马銮湾的沙氏贻贝居群之间的遗传距离为（0.276 4），深圳湾和马銮湾居群之间的遗传距离为0.306 7，深圳湾和东山湾居群之间的遗传距离为0.330。 5.说明东山种群与马銮种群之间的沙氏贻贝遗传距离较近，而深圳种群与东山种群之间的遗传距离较远。对马銮湾沙氏贻贝的18S rDNA进行测序，并与基因库中其他3个沙氏贻贝科物种进行比对，构建分子系统发育树。四个种中的紫贻贝 (Mytilopsis leucophaeata)。
We use the RAPD(random amplified polymorphic DNA) technique to analyze the gene-tic differences among three populations of Mytilopsis sallei in three bays.The results showed that the genetic distance of Mytilopsis sallei between populations of Dongshan Bay and Maluan Bay was(0.276 4),while populaitons of Shenzhen Bay and Maluan Bay was 0.306 7,and populations of Shenzhen Bay and Dongshan Bay was 0.330 5.It means that the genetic distance of Mytilopsis sallei between Dongshan population and Maluan population was closer and the genetic distance of Mytilopsis sallei between Shenzhen population and Dongshan population was farther.The 18S rDNA of Mytilopsis sallei in Maluan Bay was sequenced and compared with other three species of Dreissenidae in gene bank and the molecular phylogenetic tree is constructed.The results showed that it is much homologous between Mytilopsis sallei and Mytilopsis leucophaeata among the four species.