The Protective Effects of Piceatannol from Passion Fruit (Passiflora edulis) Seeds in UVB-Irradiated Keratinocytes
The Protective Effects of Piceatannol from Passion Fruit (Passiflora edulis) Seeds in UVB-Irradiated Keratinocytes
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DOI：
10.1248/bpb.b12-00708
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发表时间：
2013-05-01
影响因子：
2
通讯作者：
Ito, Tatsuhiko
中科院分区：
文献类型：
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作者：
Maruki-Uchida, Hiroko;Kurita, Ikuko;Ito, Tatsuhiko
使用具有显著抗氧化活性的天然植物来防止光老化正受到越来越多的关注。我们以前已经确定了云杉醇和scirpusin B，这是云杉醇的二聚体，作为强抗氧化剂，存在于西番莲（Passifiora edulis）种子。在本研究中，研究了西番莲种子提取物、云杉醇和Scirpusin B对人角质形成细胞的影响。百香果种子提取物和piceatannol以剂量依赖性方式上调角质形成细胞中的谷胱甘肽（GSH）水平，表明piceatannol是百香果种子提取物在角质形成细胞中的活性成分。白藜芦醇预处理也抑制UVB诱导的角质形成细胞中活性氧（ROS）的产生。此外，从UVB照射的角质形成细胞的介质转移到非照射的成纤维细胞增强基质金属蛋白酶（MMP）-1活性，并且当角质形成细胞用云杉醇预处理时，这种MMP-1诱导减少。这些结果表明，云杉烷醇减弱UVB诱导的MMP-1的活性，沿着角质形成细胞中ROS产生的减少。因此，含有高量的白藜芦醇的白藜芦醇和西番莲籽提取物是潜在的抗光老化化妆品成分。
The use of naturally occurring botanicals with substantial antioxidant activity to prevent photoageing is receiving increasing attention. We have previously identified piceatannol and scirpusin B, which is a dimer of piceatannol, as strong antioxidants that are present in passion fruit (Passifiora edulis) seeds. In the present study, the effects of passion fruit seed extract, piceatannol, and scirpusin B on human keratinocytes were investigated. The passion fruit seed extract and piceatannol upregulated the glutathione (GSH) levels in keratinocytes in a dose-dependent manner, indicating that piceatannol is an active component of the passion fruit seed extract in keratinocytes. The pretreatment with piceatannol also supressed the UVB-induced generation of reactive oxygen species (ROS) in the keratinocytes. In addition, the transfer of the medium from the UVB-irradiated keratinocytes to non-irradiated fibroblasts enhanced matrix-metalloproteinase (MMP)-1 activity, and this MMP-1 induction was reduced when the keratinocytes were pretreated with piceatannol. These results suggest that piceatannol attenuates the UVB-induced activity of MMP-1 along with a reduction of ROS generation in keratinocytes. Thus, piceatannol and passion fruit seed extract containing high amounts of piceatannol are potential anti-photoageing cosmetic ingredients.