Molecular Epidemiological and Serological Studies of Hantavirus Infection in Northern Vietnam
Molecular Epidemiological and Serological Studies of Hantavirus Infection in Northern Vietnam
复制标题
DOI：
10.1292/jvms.001357
复制
发表时间：
2009-10-01
影响因子：
1.2
通讯作者：
Arikawa, Jiro
中科院分区：
文献类型：
--
作者：
Truong, Thua Thang;Yoshimatsu, Kumiko;Arikawa, Jiro
对越南北方人类和啮齿动物中抗汉坦病毒抗体的分布进行了检测。用ELISA、IFA和Western blot分析方法对来自6个不同地区的健康人（617）和发热患者（220）的837份血清样品进行了抗汉滩或首尔病毒（SEOV）的IgG抗体筛查。7/617（1.1%）健康献血者、海丰港的51150名港口工人和2/185的河南省居民的血清抗体阳性。相比之下，在Ha Nam省（1/58）和清和省（4/146）的5/220（2.3%）发热患者中检测到阳性血清。抗体阳性的褐家鼠分布在河南省（7/52）、清和省（1/67）、河内的海巴统区（7/43）和海丰港（21/62）;在海丰港发现鼠（2/17）。用RT-PCR方法扩增病毒基因组中的部分Gc区。norvegicus在Haibatrung（2/7）和海丰港（7/9），票据不是来自R.家鼠（0/2）。病毒序列位于SEOV进化枝中，并与印度尼西亚SEOV形成单一谱系。这表明越南SEOV是亚洲SEOV中独特谱系的一部分。
The distribution of anti-hantavirus antibodies in humans and rodents in northern Vietnam was examined. In total, 837 seruml samples from healthy humans (617) and patients with fever (220), living in six different areas were screened for IgG antibodies against Hantaan or Seoul virus (SEOV) by ELISA, IFA, and Western blot analysis. Antibody-positive sera were identified ill 7/617 (1.1%) healthy donors, 51150 port workers in the port of Hai Phong, and 2/185 residents of Ha Nam Province. In comparison, positive sera were detected in 5/220 (2.3%) fever patients ill the provinces of Ha Nam (1/58) and Thanh Hoa (4/146). Antibody-positive Rattus norvegicus were found in the provinces of Ha Nam (7/52) and Thanh Hoa (1/67), in Haibatrung District (7/43) in Hanoi, and in Hai Phong" Port (21/62), while antibody-positive R. rattus (2/17) were found in Hai Phong Port. Part of the Gc region from the viral genome was amplified by RT-PCR using lung tissue samples front R. norvegicus in Haibatrung (2/7) and Hai Phong Port (7/9), bill not from R. rattus (0/2). Viral sequences were located in the SEOV clade and formed a single lineage with Indonesian SEOV. suggesting that Vietnamese SEOV is part of a distinct lineage among Asian SEOVs.