Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling.
Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling.
复制标题
DOI：
10.1038/s41598-022-04804-z
复制
发表时间：
2022-01-17
影响因子：
4.6
通讯作者：
Bruheim P
中科院分区：
文献类型：
--
作者：
Jang M;Scheffold J;Røst LM;Cheon H;Bruheim P
体外骨骼肌细胞生产正在成为人工实验室培育肉类领域的未来替代食品。目前，迫切需要向血清替代培养系统转变。令人惊讶的是，很少有人知道无血清培养对骨骼肌细胞的影响。因此，我们在无血清培养基（B27，AIM-V）中对C2 C12成肌细胞和肌管进行代谢分析，并将其与常规血清补充培养进行比较。此外，观察细胞形态，活力和肌原性分化培养7天。有趣的是，细胞状态之间的代谢差异比培养基影响更占主导地位。此外，在不同的培养条件下，增殖的成肌细胞比分化的肌管表现出更明显的代谢差异。与成肌细胞相比，肌管中GL 3 P和UDP-GlcNAc的细胞内水平显著增加。血清、B27和AIM-V培养物中非必需氨基酸和丙酮酸还原和转氨作用存在显著差异。细胞内代谢产物谱表明，C2 C12肌管培养在血清和B27中分别具有主要的糖酵解和氧化代谢，表明快速和慢速类型的肌肉确认的MHC免疫染色。这一工作有助于了解骨骼肌细胞在无血清培养中代谢的变化，并有助于未来人造肉的研究工作。
In vitro skeletal muscle cell production is emerging in the field of artificial lab-grown meat as alternative future food. Currently, there is an urgent paradigm shift towards a serum replacement culture system. Surprisingly, little is known about the impact of serum-free culture on skeletal muscle cells to date. Therefore, we performed metabolic profiling of the C2C12 myoblasts and myotubes in serum-free mediums (B27, AIM-V) and compared it with conventional serum supplementation culture. Furthermore, cell morphology, viability, and myogenic differentiation were observed for 7 days of cultivation. Intriguingly, the metabolic difference is more dominant between the cell status than medium effects. In addition, proliferative myoblast showed more distinct metabolic differences than differentiated myotubes in different culture conditions. The intracellular levels of GL3P and UDP-GlcNAc were significantly increased in myotubes versus myoblast. Non-essential amino acids and pyruvate reduction and transamination showed significant differences among serum, B27, and AIM-V cultures. Intracellular metabolite profiles indicated that C2C12 myotubes cultured in serum and B27 had predominant glycolytic and oxidative metabolism, respectively, indicating fast and slow types of muscle confirmed by MHC immunostaining. This work might be helpful to understand the altered metabolism of skeletal muscle cells in serum-free culture and contribute to future artificial meat research work.
登录
查看更多内容
影响因子：
6.4
作者：
Kumar K;Venkatraman V;Bruheim P
通讯作者：
Bruheim P
影响因子：
46.9
作者：
Guijas C;Montenegro-Burke JR;Warth B;Spilker ME;Siuzdak G
通讯作者：
Siuzdak G
影响因子：
5.5
作者：
Abdelmoez, Ahmed M.;Puig, Laura Sardon;Pillon, Nicolas J.
通讯作者：
Pillon, Nicolas J.
影响因子：
2.7
作者：
Lawson, MA;Purslow, PP
通讯作者：
Purslow, PP
影响因子：
5.4
作者：
Hwang, Seong Yeon;Kang, Yong Jung;Kim, Nam Deuk
通讯作者：
Kim, Nam Deuk