Sterigmatocystin production on complex and defined substrates.
Sterigmatocystin production on complex and defined substrates.
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在复杂且确定的底物上生产杂色曲霉素。
DOI：
10.1007/bf00443827
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发表时间：
1989
期刊：
影响因子：
5.5
通讯作者：
Grossman,K
中科院分区：
文献类型：
--
作者：
Bennett,JW;Henderberg,A;Grossman,K
以构巢曲霉和A.在含天冬酰胺的化学成分确定的低盐液体培养基（RLSM）、2种化学成分确定的液体培养基（AM和CZPKS）、半合成液体培养基和5种复合固体培养基（面包屑、玉米粉、燕麦、大米和小麦丝）上进行了多色培养。通过TLC鉴别并定量ST。在固体基质上，A. versicolor（M1004）在燕麦上最低（380 µg/g），在面包屑上最高（9600 µg/g），而A. nidulans（NRRL A-24747）在面包屑上产生最低的ST产量（26 µg/g），在切碎的小麦上产生最高的ST产量（80 µg/g）。培养15 d后，AM或RLSM上的两种物种均未检测到ST。在检测的确定成分培养基上，两种菌种几乎不产生ST。另外的A. versicolor（3 strs.）和A.在RLSM和AM培养基上检测了5个nidulans，但未检测到ST。
Sterigmatocystin (ST) production byAspergillus nidulansandA. versicolorcultured on a chemically defined low salts liquid medium containing asparagine (RLSM), 2 other chemically defined liquid media (AM and CZPKS), a semisynthetic liquid medium and 5 complex solid substrates (bread crumbs, cornmeal, oats, rice and shredded wheat) was studied. ST was identified and quantified by TLC. On solid substrates, yields of ST byA. versicolor(M1004) were lowest on oats (380 µg/g) and highest on breadcrumbs (9600 µg/g), whileA. nidulans(NRRL A-24747) produced the lowest ST yields on bread crumbs (26 µg/g) and the highest on shredded wheat (80 µg/g). No ST was detected for either species on AM or RLSM after 15 d in culture. Little or no ST was produced by either species on the defined media tested. Additional toxinogenic strains ofA. versicolor(3 strs.) andA. nidulans(5) were tested on RLSM and AM media, but no ST was detected.