Cytokine-induced killer cells interact with tumor lysate-pulsed dendritic cells via CCR5 signaling
Cytokine-induced killer cells interact with tumor lysate-pulsed dendritic cells via CCR5 signaling
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DOI：
10.1016/j.canlet.2016.05.020
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发表时间：
2016-08-10
期刊：
影响因子：
9.7
通讯作者：
Han, Sang-Bae
中科院分区：
文献类型：
--
作者：
Lee, Hong Kyung;Kim, Yong Guk;Han, Sang-Bae
The antitumor activity of cytokine-induced killer (CIK) cells can be increased by co-culturing them with tumor lysate-pulsed dendritic cells (tDCs); this phenomenon has been studied mainly at the populadon level. Using time-lapse imaging, we examined how CIK cells gather information from tDCs at the single-cell level. tDCs highly expressed CCL5, which bound CCR5 expressed on CIK cells. tDCs strongly induced migration of Ccr54(+/+) CIK cells, but not that of Ccr5(-/-) CIK cells or Ccr5(+/+) CIK cells treated with the CCR5 antagonist Maraviroc. Individual tDCs contacted Ccr(+/+) CIK cells more frequently and lengthily than with Ccr5(-/-) CIK cells. Consequently, tDCs increased the antitumor activity of Ccr5(+/+) CIK cells in vitro and in vivo, but did not increase that of Ccr5(-/-) CIK cells. Taken together, our data provide insight into the mechanism of CIK cell activation by tDCs at the single-cell level. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
