EPITOPE SPECIFICITY OF H-2K(B)-RESTRICTED, HSV-1-CROSS-REACTIVE, AND HSV-2-CROSS-REACTIVE CYTOTOXIC T-LYMPHOCYTE CLONES
EPITOPE SPECIFICITY OF H-2K(B)-RESTRICTED, HSV-1-CROSS-REACTIVE, AND HSV-2-CROSS-REACTIVE CYTOTOXIC T-LYMPHOCYTE CLONES
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DOI：
10.1006/viro.1993.1346
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发表时间：
1993-07-01
期刊：
影响因子：
3.7
通讯作者：
TEVETHIA, SS
中科院分区：
文献类型：
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作者：
BONNEAU, RH;SALVUCCI, LA;TEVETHIA, SS
HSV-1特异性和HSV-1/HSV-2交叉反应性H-2Kb限制性细胞毒性T淋巴细胞（CTL）克隆来源于从HSV-1感染的C57 BL/6小鼠获得的脾记忆CTL（CTLm）库。两个HSV-1/HSV-2交叉反应性CTL克隆识别HSV gB，因为用表达HSV糖蛋白B（gB）的重组腺病毒载体感染的H-2b细胞为这些CTL克隆提供了靶。使用合成肽将CTL识别表位精确地定义为HSV-1 gB残基498-505，并符合预测的H-2Kb结合基序。用对应于该预测的最小表位（HSV-1 gB 498 -505）的游离合成肽免疫C57 BL/6小鼠导致在腘淋巴结中产生HSV-gB表位特异性CD 8 +CTL。肽诱导的CTL识别并裂解HSV-1感染的H-2b细胞或用合成肽gB 498 -505脉冲的细胞。针对该预测的最小HSV CTL表位的CTL克隆的可用性将有助于理解HSV糖蛋白B的加工和该CTL识别表位的呈递。
HSV-1-specific and HSV-1/HSV-2-cross-reactive H-2Kb-restricted cytotoxic T lymphocyte (CTL) clones were derived from a pool of splenic memory CTL (CTLm) obtained from HSV-l-infected C57BL/6 mice. Two of the HSV-1/HSV-2-cross-reactive CTL clones recognized HSV gB since H-2bcells infected with a recombinant adenovirus vector expressing HSV glycoprotein B (gB) provided a target for these CTL clones. The CTL recognition epitope was precisely defined as HSV-1 gB residues 498-505 using synthetic peptides and conforms to a predicted H-2Kb-binding motif. Immunization of C57BL/6 mice with the free synthetic peptide corresponding to this predicted minimal epitope (HSV-1 gB498-505) resulted in the generation of HSV-gB epitope-specific CD8+CTL in the popliteal lymph nodes. The peptide-induced CTL recognize and lyse HSV-1 infected H-2bcells or cells pulsed with the synthetic peptide, gB498-505. The availability of CTL clones directed to this predicted minimal HSV CTL epitope should be helpful in understanding processing of HSV glycoprotein B and presentation of this CTL recognition epitope.