Facile Synthesis of Water-Soluble Zn-Doped AgIn5S8/ZnS Core/Shell Fluorescent Nanocrystals and Their Biological Application
Facile Synthesis of Water-Soluble Zn-Doped AgIn5S8/ZnS Core/Shell Fluorescent Nanocrystals and Their Biological Application
复制标题
水溶性掺锌AgIn5S8/ZnS核/壳荧光纳米晶的简易合成及其生物学应用
DOI：
10.1021/ic502600u
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发表时间：
2015-02-16
影响因子：
4.6
通讯作者：
Xu, Xiaoliang
中科院分区：
文献类型：
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作者：
Song, Jiangluqi;Jiang, Tongtong;Xu, Xiaoliang
在这里，我们展示了一种新的和简单的策略，高度发光的水溶性Zn掺杂的AgIn 5S 8（ZAIS）纳米晶体和ZAIS/ZnS核/壳结构，这是基于相应的金属和硫化物前体的乙酸盐之间的水热反应在l-半胱氨酸的存在下，在110摄氏度的聚四氟乙烯内衬高压釜。通过调整[Ag]/[Zn]的化学计量比，可以方便地将光致发光（PL）发射波长从560 nm调谐到650 nm。所制备的纳米晶体进行了系统的表征，并表现出长的PL寿命超过100 ns。考察了L-半胱氨酸浓度、反应温度等实验条件对反应的影响。此外，我们进行了一个涂层程序与ZnS壳外的ZAIS核心，并表现出优异的PL量子产率高达35%。体外细胞毒性实验表明，该材料具有较低的细胞毒性和良好的生物相容性，显示了其良好的前景。此外，所获得的ZAIS/ZnS纳米复合物（NCs）共价结合到甲胎蛋白抗体和肝癌细胞的靶向荧光成像实现。
Here we demonstrate a novel and facile strategy of highly luminescent water-soluble Zn-doped AgIn5S8 (ZAIS) nanocrystals and ZAIS/ZnS core/shell structures, which were based on hydrothermal reaction between the acetate salts of the corresponding metals and sulfide precursor in the presence of l-cysteine at 110 degrees C in a Teflon-lined autoclave. The photoluminescent (PL) emission wavelength can be conveniently tuned from 560 to 650 nm by tailoring the stoichiometric ratio of [Ag]/[Zn]. The as prepared nanocrystals were characterized systematically and exhibit long PL lifetimes more than 100 ns. The influence of experimental conditions, including concentration of l-cysteine and reaction temperature, was investigated. In addition, we performed a coating procedure with the ZnS shell outside the ZAIS core and showed excellent PL quantum yields up to 35%. The in vitro experiment exhibited quite low cytotoxicity and marvelous biocompatibility, revealing their promising prospect in bioscience. Furthermore, the obtained ZAIS/ZnS nanocompounds (NCs) were covalently conjugated to alpha-fetoprotein antibodies and targeted fluorescent imaging for hepatocellular carcinoma cells was realized.