A critical evaluation of the purification of biominerals by hypochlorite treatment.
A critical evaluation of the purification of biominerals by hypochlorite treatment.
复制标题
通过次氯酸盐处理纯化生物矿物的关键评估。
DOI：
10.1002/jbm.820270211
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Eanes,ED
中科院分区：
文献类型：
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作者：
Tomazic,BB;Brown,WE;Eanes,ED
通过次氯酸盐和肼提取对手术移植的心脏瓣膜生物假体中的钙化沉积物进行定量脱蛋白，以确定哪种是制备适合详细化学和结构表征的纯化矿物质的更好程序。次氯酸盐处理产生的材料具有比未经处理的沉积物更高的 Ca/PO4 比率。肼处理没有产生这样的效果。对钙化沉积物 X 射线衍射图的系统比较表明，与天然材料相比，次氯酸盐处理的结晶度有所增加，而肼处理的材料的结晶度没有变化。次氯酸盐处理的另一个结果是，充分研磨的钙化沉积物明显分解成尺寸范围为 50-300 nm 的颗粒群，如扫描电子显微镜所示。当这两种处理方法应用于其他生物磷灰石时，也获得了与上述结果相当的结果。另一方面，无论使用哪种脱蛋白处理，矿物质溶解度都是相当的。本研究的主要结论是，在从生物修复材料中分离病理性矿物沉积物以供进一步研究时，肼脱蛋白优于次氯酸盐提取。 © 1993 约翰威利父子公司。
The quantitative deproteination of calcific deposits from surgically explanted heart valve bioprostheses was carried out by both hypochlorite and hydrazine extraction to establish which is the better procedure for preparing purified mineral suitable for detailed chemical and structural characterization. Hypochlorite treatment resulted in a material with a higher Ca/PO4ratio than that of the untreated deposits. The hydrazine treatment did not produce such an effect. A systematic comparison of x‐ray diffraction patterns of calcific deposits showed an increase in crystallinity of hypochlorite‐treated versus native material, while the crystallinity of hydrazine‐treated materials did not change. One other result of the hypochlorite treatment was a pronounced disaggregation of well‐ground calcific deposits into a particle populations ranging from 50–300 nm in size, as shown by scanning electron microscopy. Results comparable to the above findings were also obtained when the two treatments were applied to other bioapatites. On the other hand, mineral solubilities were comparable, regardless of which deprotenation treatment was used. The principal conclusion from this study is that hydrazine deproteination is preferable to hypochlorite extraction in isolating pathologic mineral deposits from bioprosthetic materials for further study. © 1993 John Wiley & Sons, Inc.