Down-regulation of nucleophosmin/B23 during retinoic acid-induced differentiation of human promyelocytic leukemia HL-60 cells
Down-regulation of nucleophosmin/B23 during retinoic acid-induced differentiation of human promyelocytic leukemia HL-60 cells
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DOI：
10.1038/sj.onc.1201615
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发表时间：
1998-02-19
期刊：
影响因子：
8
通讯作者：
Yung, BYM
中科院分区：
文献类型：
--
作者：
Hsu, CY;Yung, BYM
通过用视黄酸（RA，10μM，1-5天）处理，诱导人早幼粒细胞白血病HL-60细胞进行粒细胞分化。在 RA 诱导的分化过程中，核磷蛋白/B23 mRNA 的稳态水平下降。在 RA 诱导的分化过程中，细胞总核磷蛋白/B23 蛋白的水平也有所下降。稳定性和核连续分析表明，RA 处理的 HL-60 细胞中核磷蛋白/B23 mRNA 的减少受到转录调节。与 c-myc mRNA 不同，在血清饥饿引起的生长停滞期间，核磷蛋白/B23 mRNA 实际上没有下降。因此，视黄酸处理后 HL-60 细胞中核磷蛋白/B23 mRNA 表达的降低可归因于细胞分化，而不是 RA 诱导的生长停滞。 Nucleophosmin/B23 反义寡聚物处理显着增强 RA 诱导的细胞分化。这项研究的结果表明，核磷蛋白/B23 是细胞分化的核仁功能下调的关键元素之一。
Human promyelocytic leukemia HL-60 cells were induced to undergo granulocytic differentiation by treatment with retinoic acid (RA, 10 mu M, 1-5 days). The steady-state level of nucleophosmin/B23 mRNA decreased during the RA-induced differentiation. There was also decrease in the level of total cellular nucleophosmin/B23 protein during the RA-induced differentiation. Stabilization and nuclear run-on assays indicate that the decrease in nucleophosmin/B23 mRNA in RA-treated HL-60 cells was transcriptionally regulated. Unlike c-myc mRNA, there was virtually no decline of nucleophosmin/B23 mRNA during the growth arrest by serum-starvation. The decrease in nucleophosmin/B23 mRNA expression in HL-60 cells subsequent to retinoic acid treatment can thus be attributed to cellular differentiation rather than the growth arrest induced by RA. Nucleophosmin/B23 antisense oligomer treatment significantly potentiated RA-induced cellular differentiation. Results of this study suggest that nucleophosmin/B23 is one of the key elements in the down-regulation of nucleolar function for cellular differentiation.