Existence of trichohyalin-keratohyalin hybrid granules: co-localization of two major intermediate filament-associated proteins in non-follicular epithelia.
Existence of trichohyalin-keratohyalin hybrid granules: co-localization of two major intermediate filament-associated proteins in non-follicular epithelia.
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毛透明蛋白-角质透明蛋白混合颗粒的存在：非滤泡上皮中两种主要中间丝相关蛋白的共定位。
DOI：
10.1046/j.1432-0436.1994.5810065.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
O'Guin,WM
中科院分区：
文献类型：
--
作者：
Manabe,M;O'Guin,WM
毛透明蛋白是一种相对高分子量（‐200 kDa）的蛋白质，与中间丝有关，多年来被认为仅在毛囊的内根鞘和髓质中表达。然而，我们在这里表明，这种蛋白与聚丝蛋白在许多非滤泡、角质化、分层上皮的颗粒层中表达相关，这些上皮也表达角蛋白K6/K16，包括舌背上皮的丝状乳头。在这种上皮中，在其上层细胞层中形成形态不均匀的角透明蛋白颗粒，毛透明蛋白与聚丝蛋白形成杂交颗粒，聚丝蛋白是角透明蛋白颗粒中发现的主要中间丝相关蛋白，通常在表皮分化晚期表达。这两种中间的丝相关蛋白在杂交颗粒中保持物理分离，但它们的命运相同，因为它们都分散在移行细胞中，并且在角化细胞中无法检测到。甲基质上皮、胸腺Hassal小体上皮和新生包皮表皮中也检测到毛透明素。它在正常的躯干和头皮表皮中基本不存在，但在颗粒层的一些分散的细胞中表达，这些细胞也是聚丝蛋白阳性的。此外，在许多增生性皮肤病中，毛透明蛋白在表皮中表达。这些发现表明，毛透明蛋白不是少数毛囊细胞所特有的，而是在许多正常和病理上皮中表达的，其中它与聚丝蛋白独特相关。此外，由于所有这些表达毛透明蛋白的角质形成细胞也合成角蛋白K6和K16（角质形成细胞分化的“替代”途径的标记），这提出了毛透明蛋白特异性（或优先）参与聚集含有K6/K16角蛋白的中间细丝的可能性。
Trichohyalin is a protein of relatively high molecular weight (‐200 kDa), associated with intermediate filaments, that was for many years thought to be expressed only in the inner root sheath and medulla of the hair follicle. We show here, however, that this protein is expressed in association with (pro)filaggrin in the granular layer of many non‐follicular, keratinizing, stratified epithelia which also express keratins K6/K16, including those of the filiform papillae of dorsal tongue epithelia. In this epithelium, which elaborates morphologically heterogeneous keratohyalin granules in its upper cell layers, trichohyalin forms hybrid granules with filaggrin, the major intermediate filament associated protein found in keratohyalin granules, which is normally expressed in advanced epidermal differentiation. These two intermediate filament‐associated proteins remain physically segregated in the hybrid granules, but they share the same fate, as they both become dispersed in transitional cells, and are undetectable in cornified cells. Trichohyalin was also detected in nail matrix epithelia, the epithelium of Hassal's corpuscles of the thymus, and newborn foreskin epidermis. It is essentially absent from normal trunk and scalp epidermis, but is expressed in a few scattered cells of the granular layer that are also filaggrin‐positive. In addition, trichohyalin is expressed in the epidermis in a number of hyperplastic skin diseases. These findings demonstrate that trichohyalin is not peculiar to a small number of hair follicle cells, but is expressed in a number of normal and pathological epithelia where it is uniquely associated with filaggrin. In addition, since all these trichohyalin‐expressing keratinocytes also synthesize keratins K6 and K16 (the markers for an “alternative” pathway of keratinocyte differentiation), this raises the possibility that the trichohyalin protein is specifically (or preferentially) involved in aggregating intermediate filaments containing the K6/K16 keratins.
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