Novel kojic acid-polymer-based magnetic nanocomposites for medical applications.
Novel kojic acid-polymer-based magnetic nanocomposites for medical applications.
复制标题
DOI：
10.2147/ijn.s53847
复制
发表时间：
2014
影响因子：
8
通讯作者：
Webster TJ
中科院分区：
文献类型：
--
作者：
Hussein-Al-Ali SH;El Zowalaty ME;Hussein MZ;Ismail M;Dorniani D;Webster TJ
采用氢氧化钠溶液共沉淀法制备了氧化铁磁性纳米粒子（MNP），并分别用壳聚糖（CS）和聚乙二醇（PEG）包覆，制备了CS-MNP和PEG-MNP纳米粒子。然后将它们与具有生物活性的天然化合物曲酸（KA）负载，分别形成KA-CS-MNP和KA-PEG-MNP纳米复合物。采用粉末X射线衍射、傅里叶变换红外光谱、热重分析、振动样品磁强计和扫描电子显微镜对MNP及其纳米复合材料进行了表征。粉末X-射线衍射数据表明，所有制剂由高度结晶的纯磁铁矿Fe 3 O 4组成。傅里叶变换红外光谱和热重分析证实了聚合物和KA的纳米复合材料中的存在。磁化曲线表明，KA-CS-MNP和KA-PEG-MNP均为超顺磁性材料，饱和磁化强度分别为8.1 emu/g和26.4 emu/g。使用紫外-可见光谱估计KA药物负载，其分别给出KA-CS-MNP和KA-PEG-MNP纳米复合物的12.2%和8.3%的负载。KA从纳米复合材料中的释放曲线遵循伪二级动力学模型。进行琼脂扩散测试以使用两种革兰氏阳性（耐甲氧西林金黄色葡萄球菌和枯草芽孢杆菌）和一种革兰氏阴性（肠道沙门氏菌）物种来评估KA-CS-MNP和KA-PEG-MNP纳米复合物针对多种微生物的抗微生物活性，并且显示出一定的抗微生物活性，其可以在未来的研究中通过优化药物负载来增强。该研究为进一步研究KA以及KA-CS-MNP和KA-PEG-MNP纳米复合材料在纳米药物应用中可能的有益生物活性提供了证据。
Iron oxide magnetic nanoparticles (MNPs) were synthesized by the coprecipitation of iron salts in sodium hydroxide followed by coating separately with chitosan (CS) and polyethylene glycol (PEG) to form CS-MNPs and PEG-MNPs nanoparticles, respectively. They were then loaded with kojic acid (KA), a pharmacologically bioactive natural compound, to form KA-CS-MNPs and KA-PEG-MNPs nanocomposites, respectively. The MNPs and their nanocomposites were characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, vibrating sample magnetometry, and scanning electron microscopy. The powder X-ray diffraction data suggest that all formulations consisted of highly crystalline, pure magnetite Fe3O4. The Fourier transform infrared spectroscopy and thermogravimetric analysis confirmed the presence of both polymers and KA in the nanocomposites. Magnetization curves showed that both nanocomposites (KA-CS-MNPs and KA-PEG-MNPs) were superparamagnetic with saturation magnetizations of 8.1 emu/g and 26.4 emu/g, respectively. The KA drug loading was estimated using ultraviolet–visible spectroscopy, which gave a loading of 12.2% and 8.3% for the KA-CS-MNPs and KA-PEG-MNPs nanocomposites, respectively. The release profile of the KA from the nanocomposites followed a pseudo second-order kinetic model. The agar diffusion test was performed to evaluate the antimicrobial activity for both KA-CS-MNPs and KA-PEG-MNPs nanocomposites against a number of microorganisms using two Gram-positive (methicillin-resistant Staphylococcus aureus and Bacillus subtilis) and one Gram-negative (Salmonella enterica) species, and showed some antibacterial activity, which could be enhanced in future studies by optimizing drug loading. This study provided evidence for the promise for the further investigation of the possible beneficial biological activities of KA and both KA-CS-MNPs and KA-PEG-MNPs nanocomposites in nanopharmaceutical applications.