Genetic mapping and genome-wide association study identify BhYAB4 as the candidate gene regulating seed shape in wax gourd (Benincasa hispida).
Genetic mapping and genome-wide association study identify BhYAB4 as the candidate gene regulating seed shape in wax gourd (Benincasa hispida).
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DOI：
10.3389/fpls.2022.961864
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
冬瓜是葫芦科重要的蔬菜作物。根据种皮的形状和结构，冬瓜种子可分为双侧和单侧。双边种子通常发芽快，发芽率比单边种子高。因此，双边种子冬瓜品种更受到种子公司和种植户的欢迎。然而，冬瓜种子形状调控的遗传基础和分子机制仍不清楚。本研究遗传分析表明，冬瓜种子形状由单一基因控制，由双侧显性变为单侧显性。结合遗传图谱和全基因组关联研究，将编码YABBY转录因子的Bhi04G000544（BhYAB4）确定为冬瓜种子形状测定的候选基因。在不同种质资源中检测到BhYAB4的G/A单核苷酸多态性变异，BhYAB4G在双侧种子中特异富集，BhYAB4A在单侧种子中特异富集。 G 到 A 的突变导致 BhYAB4 的内含子保留和提前终止密码子。表达分析表明，BhYAB4G和BhYAB4A在种子中均高表达，但与BhYAB4G蛋白相比，BhYAB4A蛋白的核定位受到干扰。最后，开发了一种可有效区分双边和单边种子的衍生切割扩增多态性序列标记，从而促进了冬瓜品种的分子标记辅助育种。
Wax gourd is an important vegetable crop of the Cucurbitaceae family. According to the shape and structure of the seed coat, the seeds of the wax gourd can be divided into bilateral and unilateral. Bilateral seeds usually germinate quickly and have a high germination rate than unilateral seeds. Thereby, wax gourd varieties with bilateral seeds are more welcomed by seed companies and growers. However, the genetic basis and molecular mechanism regulating seed shape remain unclear in the wax gourd. In this study, the genetic analysis demonstrated that the seed shape of wax gourd was controlled by a single gene, with bilateral dominant to unilateral. Combined with genetic mapping and genome-wide association study, Bhi04G000544 (BhYAB4), encoding a YABBY transcription factor, was identified as the candidate gene for seed shape determination in the wax gourd. A G/A single nucleotide polymorphism variation of BhYAB4 was detected among different germplasm resources, with BhYAB4G specifically enriched in bilateral seeds and BhYAB4A in unilateral seeds. The G to A mutation caused intron retention and premature stop codon of BhYAB4. Expression analysis showed that both BhYAB4G and BhYAB4A were highly expressed in seeds, while the nuclear localization of BhYAB4A protein was disturbed compared with that of BhYAB4G protein. Finally, a derived cleaved amplified polymorphic sequence marker that could efficiently distinguish between bilateral and unilateral seeds was developed, thereby facilitating the molecular marker-assisted breeding of wax gourd cultivars.
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影响因子：
0.4
作者：
Han, H. Q.;Liu, Y.;Chen, H. Y.
通讯作者：
Chen, H. Y.
影响因子：
5.3
作者：
Li MX;Yeung JM;Cherny SS;Sham PC
通讯作者：
Sham PC
利用特定位点扩增片段（SLAF）测序技术构建冬瓜果皮颜色高密度遗传图谱及基因定位
影响因子：
4.4
作者：
Jiang B;Liu W;Xie D;Peng Q;He X;Lin Y;Liang Z
通讯作者：
Liang Z
影响因子：
11.6
作者：
Klucher, KM;Chow, H;Fischer, RL
通讯作者：
Fischer, RL
影响因子：
9.2
作者：
Eshed, Y;Baum, SF;Bowman, JL
通讯作者：
Bowman, JL