Mapping of gene-specific markers on the genetic map of chickpea (Cicer arietinum L.)
Mapping of gene-specific markers on the genetic map of chickpea (Cicer arietinum L.)
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DOI：
10.1007/s00438-003-0828-0
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发表时间：
2003-05-01
影响因子：
3.1
通讯作者：
Kahl, G
中科院分区：
文献类型：
--
作者：
Pfaff, T;Kahl, G
鹰嘴豆（Cicer arietinum L.）不明为了增加我们对该基因组基本结构的了解，我们通过遗传连锁分析确定了一系列参与植物防御反应（DR）的基因的图谱位置。利用GenBank中的序列数据，我们选择了已知在鹰嘴豆和其他植物中被病原体攻击诱导的基因。根据鹰嘴豆的保守区设计基因特异性引物，对鹰嘴豆与鹰嘴豆种间杂交的分离群体中相应的基因序列进行检测。网状细胞将47个基因特异性标记整合到基于STMS、AFLP、AFLP和其他匿名标记的现有图谱中。这种方法的潜力进行了讨论。
With the exception of the fact that it is made up of eight different chromosomes, the physical organization of the 738-Mb genome of the important legume crop chickpea (Cicer arietinum L.) is unknown. In an attempt to increase our knowledge of the basic structure of this genome, we determined the map positions of a series of genes involved in plant defence responses (DR) by genetic linkage analysis. Exploiting the sequence data available in GenBank, we selected genes known to be induced in chickpea and other plants by pathogen attack. Gene-specific primers were designed based on conserved regions, and used to detect the corresponding gene sequences in a segregating population derived from an interspecific cross between Cicer arietinum and C. reticulatum. Forty-seven gene-specific markers were integrated into an existing map based on STMS, AFLP, DAF and other anonymous markers. The potential of this approach is discussed.