Aromatic DNA adducts in relation to dietary and other lifestyle factors in Spanish adults
Aromatic DNA adducts in relation to dietary and other lifestyle factors in Spanish adults
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芳香族 DNA 加合物与西班牙成年人饮食和其他生活方式因素的关系
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
A. Agudo
中科院分区：
文献类型：
--
作者：
R. Ibáñez;M. Peluso;A. Munnia;S. Piro;C. González;P. Amiano;M. Tormo;E. Ardanaz;A. Barricarte;A. Berenguer;M. Chirlaque;M. Dorronsoro;P. Jakszyn;N. Larranaga;C. Martínez;C. Navarro;J. Quirós;M. Sanchez;A. Agudo
本研究的目的是评估欧洲癌症与营养前瞻性调查（EPIC）西班牙队列样本中白色血细胞（WBC）中大体积DNA加合物与生活方式因素之间的关系。纳入了来自5个地区的296名年龄在35至64岁之间的受试者。食物摄入量估计与计算机版本的饮食史问卷。多环芳烃（PAH）的每日摄入量估计使用的数据库与潜在致癌物的食物含量的信息。收集生活方式和健康因素的数据，并使用核酸酶P1 32 P-后标记技术测量DNA加合物。男性和女性加合物的几何平均值相似（分别为4.11/109和3.94/109个核苷酸）。在非吸烟者和非职业接触者中观察到最高水平的加合物。肉类摄入量，油和脂肪与更高水平的加合物有关，但都没有统计学意义。较高的钙、钠和磷摄入量与较低的加合物水平相关。总之，我们的研究表明，从白细胞DNA中的32 P-后标记测量的笨重的加合物与健康的西班牙成年人的日常饮食无关。虽然有人认为饮食是非吸烟人群中PAH的主要来源，但DNA加合物似乎不是一般人群中膳食PAH的合适生物标志物。
The aim of this study was to assess the relationship between bulky DNA adducts in white blood cells (WBC) and lifestyle factors in a sample from the Spanish cohort of the European Prospective Investigation into Cancer and Nutrition (EPIC). 296 subjects aged between 35 and 64 years, from five regions, were included. Food intake was estimated with a computerized version of dietary history questionnaire. Daily intake of polycyclic aromatic hydrocarbons (PAH) was estimated using a database with information on food content of potential carcinogens. Data on lifestyle and health factors were collected and DNA adducts measured using the nuclease P1 32P-postlabelling technique. Geometric means of adducts were similar for men and women (4.11/109 and 3.94/109 nucleotides, respectively). Highest levels of adduct were observed in non-smokers and non-occupationally exposed. Meat intake, oils and fats were associated with higher levels of adducts, but all non-statistically significant. Higher intakes of calcium, sodium and phosphorus were associated with lower adducts levels. Summarising, our study shows that bulky adducts measured by 32P-postlabelling in DNA from WBC do not correlate with the usual diet of healthy Spanish adults. Although it has been proposed that diet be the main source of PAH in nonsmokers without occupational exposure, DNA adducts do not seem to be suitable biomarkers of dietary PAH in general population.
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大体积芳香族加合物的 32P 标记后分析。
DOI：
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发表时间：
1993
期刊：
IARC scientific publications
影响因子：
--
作者：
Gupta,RC
通讯作者：
Gupta,RC
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DOI：
10.1016/0165-1218(90)90107-d
发表时间：
1990
期刊：
Mutation research
影响因子：
--
作者：
Reddy,MV;Randerath,K
通讯作者：
Randerath,K
影响因子：
11.2
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通讯作者：
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影响因子：
11.2
作者：
Sinha, R;Peters, U;Hayes, RB
通讯作者：
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