High-affinity substance P binding sites in rat sympathetic ganglia.
High-affinity substance P binding sites in rat sympathetic ganglia.
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大鼠交感神经节中的高亲和力 P 物质结合位点。
DOI：
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发表时间：
1985
影响因子：
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通讯作者：
J. Saavedra
中科院分区：
文献类型：
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作者：
M. Niwa;K. Shigematsu;L. Plunkett;J. Saavedra
通过将组织切片与 125I-Bolton-Hunter P 物质一起孵育、放射自显影、图像分析与计算机辅助显微密度测定相结合，并与 125I 标准品进行比较，对单只大鼠交感神经节中的 P 物质结合位点进行了表征和定量。椎前腹腔-肠系膜上交感神经节含有高密度、高亲和力 P 物质结合位点簇[最大结合 (Bmax) 7.12 +/- 0.20 pmol/mg 蛋白质；亲和常数 (Ka) 2.08 +/- 0.02 X 10(9) M-1]。在椎旁上颈交感神经节中，高亲和力 P 物质结合位点的数量明显较低（Bmax 1.38 +/- 0.04 pmol/mg 蛋白质；Ka 1.71 +/- 0.03 X 10(9) M-1）。未来的研究应该解决大鼠椎前神经节中确定的 P 物质结合位点是否充当推测由感觉神经元末梢释放的肽的受体的问题。
Substance P binding sites were characterized and quantitated in single rat sympathetic ganglia by incubation of tissue sections with 125I-Bolton-Hunter substance P, autoradiography, image analysis coupled with computer-assisted microdensitometry, and comparison with 125I standards. The prevertebral celiac-superior mesenteric sympathetic ganglia contained clusters of high-density, high-affinity substance P binding sites [maximum binding (Bmax) 7.12 +/- 0.20 pmol/mg protein; affinity constant (Ka) 2.08 +/- 0.02 X 10(9) M-1]. In the paravertebral superior cervical sympathetic ganglia, the number of high-affinity substance P binding sites was substantially lower (Bmax 1.38 +/- 0.04 pmol/mg protein; Ka 1.71 +/- 0.03 X 10(9) M-1). Future studies should address the issue of whether the substance P binding sites identified in prevertebral ganglia of the rat serve as receptors for the peptide presumed to be released by terminals of sensory neurons.