A comparative study of the dentate gyrus in hippocampal sclerosis in epilepsy and dementia.
A comparative study of the dentate gyrus in hippocampal sclerosis in epilepsy and dementia.
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DOI：
10.1111/nan.12087
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发表时间：
2014-02
影响因子：
5
通讯作者：
Thom M
中科院分区：
文献类型：
--
作者：
Bandopadhyay R;Liu JY;Sisodiya SM;Thom M
海马硬化症（HS）长期被认为与癫痫（HSE）相关，最近在老年人认知下降或痴呆（HSD）的背景下，在某些情况下作为神经退行性疾病的组成部分，包括阿尔茨海默病（AD）和额颞叶痴呆（FTLD）。在AD中癫痫发作的风险增加，在转基因AD模型的齿状回中自发性癫痫样放电;癫痫可能与AD型病理学和认知能力下降的年龄加速增加相关。这些疾病过程之间的会聚可能与海马病理学有关。HSE通常显示齿状回中兴奋性和抑制性神经元网络的重组，并且被认为与海马兴奋性相关。我们试图比较HSE和HSD的病理学，重点是齿状回的重组。研究方法：在9个死后的情况下，HSE和双边的损害，18 HSD和11个对照组，我们进行了免疫组化染色苔藓纤维（强啡肽），和神经元间网络（NPY，钙结合蛋白和calretinin）的部分从海马体中部。齿状回中的纤维发芽（FS）或表达丧失从0级（正常）到3级（显著改变）进行半定量分级。结果：HSE组四项指标的重组率均显著高于HSD组（P < 0.01）。HSD组强啡肽（3例FS）、钙网膜素（6例FS）、神经肽Y（11例FS）、钙结合素（10例丢失）轻度改变。在8例HSD病例中，观察到一种以上抗体的改变，但没有观察到最高级别。我们还注意到NPY，并在较小程度上，calretinin标记的平野体在CA 1的AD病例和一些老年人的控制，但不是在HSE。结论：齿状回兴奋性和抑制性神经网络的重组更具有HSE的特征。HSD的细微变化可能是海马兴奋性增加的结果，包括未识别的癫痫发作活动。一个意想不到的发现是在HSD而不是HSE中鉴定出NPY阳性的平野小体，这可能是这些条件下中间神经元相对脆弱的结果。
Hippocampal sclerosis (HS) is long-recognized in association with epilepsy (HSE) and more recently in the context of cognitive decline or dementia in the elderly (HSD), in some cases as a component of neurodegenerative diseases, including Alzheimer's disease (AD) and fronto-temporal lobe dementia (FTLD). There is an increased risk of seizures in AD and spontaneous epileptiform discharges in the dentate gyrus of transgenic AD models; epilepsy can be associated with an age-accelerated increase in AD-type pathology and cognitive decline. The convergence between these disease processes could be related to hippocampal pathology. HSE typically shows re-organization of both excitatory and inhibitory neuronal networks in the dentate gyrus, and is considered to be relevant to hippocampal excitability. We sought to compare the pathology of HSE and HSD, focusing on re-organization in the dentate gyrus. Methods: In nine post mortem cases with HSE and bilateral damage, 18 HSD and 11 controls we carried out immunostaining for mossy fibres (dynorphin), and interneuronal networks (NPY, calbindin and calretinin) on sections from the mid-hippocampal body. Fibre sprouting (FS) or loss of expression in the dentate gyrus was semi-quantitatively graded from grade 0 (normal) to grade 3 (marked alteration). Results: Significantly more re-organization was seen with all four markers in the HSE than HSD group (P < 0.01). Mild alterations were noted in HSD group with dynorphin (FS in 3 cases), calretinin (FS in 6 cases), NPY (FS in 11 cases) and calbindin (loss in 10 cases). In eight HSD cases, alteration was seen with more than one antibody but in no cases were the highest grades seen. We also noted NPY and, to a lesser extent, calretinin labelling of Hirano bodies in CA1 of AD cases and some older controls, but not in HSE. Conclusion: Reorganization of excitatory and inhibitory networks in the dentate gyrus is more typical of HSE. Subtle alterations in HSD may be a result of increased hippocampal excitability, including unrecognized seizure activity. An unexpected finding was the identification of NPY-positive Hirano bodies in HSD but not HSE, which may be a consequence of the relative vulnerabilities of interneurons in these conditions.
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影响因子：
4.7
作者：
Jouvenceau, A;Potier, B;Billard, JM
通讯作者：
Billard, JM
影响因子：
14.5
作者：
Liu, Joan Y. W.;Thom, Maria;Sisodiya, Sanjay M.
通讯作者：
Sisodiya, Sanjay M.
影响因子：
3.3
作者：
Bouilleret, V;Schwaller, B;Fritschy, JM
通讯作者：
Fritschy, JM
影响因子：
2.2
作者：
Martinian, L.;Catarino, C. B.;Thom, M.
通讯作者：
Thom, M.
影响因子：
2.1
作者：
Corrada, Maria M.;Berlau, Daniel J.;Kawas, Claudia H.
通讯作者：
Kawas, Claudia H.