Angiogenic activity of placental tissues of cows.
Angiogenic activity of placental tissues of cows.
复制标题
牛胎盘组织的血管生成活性。
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
D. Redmer
中科院分区：
文献类型：
--
作者：
L. Reynolds;D. S. Millaway;J. Kirsch;J. Infeld;D. Redmer
在Exp. 1，从妊娠中期的母牛获得胎盘的母体（肉阜）和胎儿（子叶）部分以及子宫内膜，并通过将组织样品置于鸡绒毛尿囊膜（CAM）上来评价血管生成活性。在CAM试验中，只有泪阜组织表现出血管生成活性。在Exp. 2、评价了从妊娠中期母牛获得的肾阜组织的冻干匀浆对CAM的血管生成活性和它们在体外刺激牛主动脉内皮细胞有丝分裂的能力。种阜组织的匀浆在CAM上再次是血管生成的，并且对于内皮细胞也是促有丝分裂的。在Exp. 3、从妊娠中期的奶牛中获得胎盘的母体（肉阜和子宫内膜）和胎儿（子叶和胎膜）部分，并将每种部分的细切碎物（外植体）培养24小时。然后通过体外刺激牛主动脉内皮细胞有丝分裂和迁移的能力来测试解释条件培养基的血管生成活性。条件培养基从caruncular外植体，但不是从其他组织的外植体，表现出促有丝分裂和迁移刺激活动。当通过超滤分离泪阜条件培养基池时，在Mr小于10，000、小于30，000和小于100，000的组分中不存在促有丝分裂活性，但在Mr大于10，000、大于30，000和大于100，000的组分中保留有促有丝分裂活性。在进行热处理的任何组分中均未观察到促有丝分裂活性。（250字处删节）
In Exp. 1, maternal (caruncle) and fetal (cotyledon) portions of the placenta as well as uterine endometrium were obtained from cows at mid-gestation and evaluated for angiogenic activity by placing tissue samples on chick chorioallantoic membranes (CAM). Only caruncular tissues exhibited angiogenic activity in the CAM assay. In Exp. 2, lyophilized homogenates of caruncular tissues obtained from cows at mid-gestation were evaluated for angiogenic activity on CAM and for their ability to stimulate mitosis of bovine aortic endothelial cells in vitro. Homogenates of caruncular tissues again were angiogenic on the CAM and also were mitogenic for endothelial cells. In Exp. 3, maternal (caruncle and endometrium) and fetal (cotyledon and fetal membrane) portions of the placenta were obtained from cows at mid-gestation and fine minces (explants) of each were cultured for 24 h. Explant-conditioned media were then tested for angiogenic activity by their abilities to stimulate mitosis and migration of bovine aortic endothelial cells in vitro. Conditioned media from caruncular explants, but not from explants of other tissues, exhibited both mitogenic and migration-stimulating activities. When pools of caruncular explant-conditioned media were fractionated by ultrafiltration, mitogenic activity was not present in fractions of Mr less than 10,000, less than 30,000 and less than 100,000, but was retained in fractions of Mr greater than 10,000, greater than 30,000 and greater than 100,000. Mitogenic activity was not observed in any fractions subjected to heat treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
禁食对绵羊子宫血流量和底物吸收的影响。
DOI：
10.1093/jn/110.12.2433
发表时间：
1980
期刊：
The Journal of nutrition
影响因子：
--
作者：
Morriss,FH;Rosenfeld,CR;Crandell,SS;Adcock3rd,EW
通讯作者：
Adcock3rd,EW
糖胺聚糖：它们在非妊娠和妊娠绵羊子宫中的分布和潜在的血管活性。
影响因子：
9.8
作者：
GreissJr,FC;Wagner,WD
通讯作者：
Wagner,WD
大鼠颗粒细胞条件培养基刺激内皮细胞增殖。
影响因子：
4.8
作者：
Koos,RD
通讯作者：
Koos,RD