Improved yield of micropropagated sugarcane following inoculation by endophytic Burkholderia vietnamiensis
Improved yield of micropropagated sugarcane following inoculation by endophytic Burkholderia vietnamiensis
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DOI：
10.1007/s11104-005-3223-2
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发表时间：
2006-02-01
期刊：
影响因子：
4.9
通讯作者：
Lakshminarasimhan, C
中科院分区：
文献类型：
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作者：
Govindarajan, M;Balandreau, J;Lakshminarasimhan, C
在泰米尔纳德邦对与甘蔗相关的固氮伯克霍尔德氏菌进行调查时，在 PCAT 培养基上分离出了一些内生菌。分离是基于使用选择性 PCAT 培养基。研究了四种分离株，它们均属于伯克霍尔德氏菌属。其中之一，MG43 在还原乙炔方面始终更加活跃，被鉴定为越南伯克霍尔德杆菌。该分离株用于接种微繁殖甘蔗幼苗，与其他两种固氮菌进行比较。固氮葡糖醋杆菌 (T) 和 Herbaspirillum seropedicae (T)。将接种的植物和未接种的对照用于盆栽实验，然后在不同氮肥施用量下进行两次田间实验。 MG43 和 G. diazotropicus 在它们的天然宿主甘蔗中表现最好。由于接种 MG43，田间生物量增加达到 20%。接种的植物大量被接种的细菌定植（根鲜重高达 115,000 CFU g(-1)）。三种菌株的组合接种效果不如单一 MG43 悬浮液接种效果好。讨论了生态影响，以及这些细菌在低投入和长期可持续农村经济中为较高氮肥施用率提供可行替代品的潜力。
During a survey of nitrogen-fixing Burkholderia associated with sugarcane in Tamil Nadu, some endophytes were isolated on PCAT medium. Isolation was based on the use of the selective PCAT medium. Four isolates were studied, all belonging to the genus Burkholderia. One of them, MG43 was consistently more active in reducing acetylene and was identified as Burkholderia vietnamiensis. This isolate was used to inoculate micro-propagated sugarcane plantlets in a comparison with two other diazoptrophs, viz. Gluconacetobacter diazotrophicus(T) and Herbaspirillum seropedicae(T). Inoculated plants and uninoculated controls were used in a pot experiment followed by two field experiments under different rates of nitrogen fertilisers. MG43 and G. diazotrophicus performed best in sugarcane, their natural host. Biomass increase due to MG43 inoculation reached 20% in the field. Inoculated plants were heavily colonised by the inoculated bacterium (up to 115,000 CFU g(-1) root fresh weight). Inoculation by a combinaison of the three strains performed less well than inoculation by a single MG43 suspension. Ecological implications are discussed, as well as the potential of these bacteria to provide a feasible alternative to higher N fertilisers rates in a low input and long term sustainable rural economy.