Detection of diarrhetic shellfish poisoning toxins using high-sensitivity human cancer cell-based impedance biosensor
Detection of diarrhetic shellfish poisoning toxins using high-sensitivity human cancer cell-based impedance biosensor
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DOI：
10.1016/j.snb.2015.08.061
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发表时间：
2016-01-01
影响因子：
8.4
通讯作者：
Wang, Ping
中科院分区：
文献类型：
--
作者：
Zou, Ling;Wang, Qin;Wang, Ping
腹泻性贝类毒素是一种严重危害人类健康和渔业生产的海产品污染物。大量的动物生物测定和化学分析方法用于DSP毒素检测。然而，这些毒素检测方法都是低通量和高成本的，这阻碍了它们的广泛应用。本研究以HeLa细胞和HepG 2细胞为敏感细胞，建立了检测DSP毒素冈田酸（okadaic acid，OA）细胞毒性的CIB。基于HeLa和HepG 2的生物传感器的检测限（LOD）分别为10.2 μ g/L和3.3 μ g/L，均低于传统的基于细胞的测定（HeLa细胞为21.2 μ g/L，HepG 2细胞为9.8 μ g/L）。从CIB获得的HeLa和HepG 2细胞中OA的半数最大抑制浓度（IC 50）值（49.9 +/- 4.9和39.2 +/- 4.3 μ g/L）均低于细胞计数试剂盒-8测定（CCK 8）（62.7 +/- 7.1和45.8 +/- 6.7 μ g/L）。此外，CIB测定与小鼠生物测定（MBA）和液相色谱串联质谱（LC-MS/MS）具有良好的相关性。综上所述，所有的结果表明，CIB技术具有很大的潜力，是一个有效的补充，在DSP毒素检测。(C)2015 Elsevier B. V.版权所有。
Diarrhetic shellfish poisoning (DSP) toxin is a dangerous contamination in seafood worldwide that can threaten human health and fishing. A large number of animal bioassays and chemical analytical methods are employed for DSP toxin detection. However, these toxin detection methods are low-throughput and high-cost which hamper their wide applications. In this study, HeLa and HepG2 cell lines were selected as the sensitive elements to establish the CIBs for monitoring the cytotoxicity induced by a representative DSP toxin, okadaic acid (OA). The limit of detection (LOD) of HeLa- and HepG2-based biosensors are 10.2 mu g/L and 3.3 mu g/L, respectively, which are both lower than the conventional cell-based assay (21.2 mu g/L of HeLa cells and 9.8 mu g/L of HepG2 cells). The half maximal inhibitory concentration (IC50) values of OA in HeLa and HepG2 cells which were obtained from CIB (49.9 +/- 4.9 and 39.2 +/- 4.3 mu g/L) are both lower than Cell Counting Kit-8 assay (CCK8) (62.7 +/- 7.1 and 45.8 +/- 6.7 mu g/L). Besides, CIB measurement presents good correlation with mouse bioassay (MBA) and liquid chromatography tandem mass spectrometry (LC-MS/MS). In summary, all the results indicate that the CIB technology had great potential to be an effective complement in DSP toxins detection. (C) 2015 Elsevier B.V. All rights reserved.