Lead intoxication: antioxidant defenses and oxidative damage in rat brain
Lead intoxication: antioxidant defenses and oxidative damage in rat brain
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DOI：
10.1016/s0300-483x(99)00051-7
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发表时间：
1999-07-15
期刊：
影响因子：
4.5
通讯作者：
Oteiza, PI
中科院分区：
文献类型：
--
作者：
Adonaylo, VN;Oteiza, PI
与铅（Pb）在脑中的存在相关的氧化损伤已被提出作为铅毒性的一个可能的机制。为了研究这一假设，我们研究了长期的影响Pb 2+对氧化应激参数在大脑中的大鼠长期暴露于金属（1克醋酸铅/升饮用水）。治疗8周后，与对照组相比，Pb 2+中毒大鼠（血铅浓度> 100 μ g/dl）显示出较低的体重、较低的红细胞压积和5-氨基乙酰丙酸脱氢酶活性。铅中毒组脑内2-硫代巴比妥酸反应物质（TBARS）含量显著高于对照组（P < 0.05）。高活性的抗氧化酶谷胱甘肽还原酶和谷胱甘肽过氧化物酶，和一个较低的水平（44%）的泛醇10被发现在大脑中的Pb 2+处理的大鼠，与对照组相比。脑泛醇9（r（2）= 0.79）、10（r（2）= 0.84）与血铅浓度呈负相关。脑α-生育酚水平，超氧化物歧化酶活性和蛋白质的氧化损伤的参数之间的控制和Pb 2+处理的大鼠相似。本研究结果表明，慢性铅中毒诱导大鼠脑氧化应激的情况。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
Oxidative damage associated with the presence of lead (Pb) in the brain has been proposed as one possible mechanism involved in Pb toxicity. To investigate this hypothesis, we examined the long-term effects of Pb2+ on parameters of oxidative stress in the brain from rats chronically exposed to the metal (1 g Pb acetate/l drinking water). After 8 weeks of treatment, Pb2+-intoxicated rats (blood Pb concentration > 100 mu g/dl) showed lower body weight, and lower hematocrit and 5-aminolevulinic acid dehydratase activity as compared to controls. The content of brain 2-thiobarbituric acid-reactive substances (TBARS), an indicator of lipid oxidation, was significantly (P < 0.05) higher in the Pb2+-intoxicated animals than in controls. Higher activities of the antioxidant enzymes glutathione reductase and glutathione peroxidase, and a lower (44%) level of ubiquinol 10 were found in the brain of the Pb2+-treated rats, compared to controls. A negative correlation between brain ubiquinol 9 (r(2) = 0.79), 10 (r(2) = 0.84) and blood Pb concentration was observed. Brain alpha-tocopherol levels, superoxide dismutase activity and parameters of oxidative damage to proteins were similar between control and Pb2+-treated rats. The present results indicate that chronic Pb2+ intoxication induces an oxidative stress situation in rat brain. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.