Hyaline cartilage: In vivo and in vitro assessment with magnetization transfer imaging
Hyaline cartilage: In vivo and in vitro assessment with magnetization transfer imaging
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DOI：
10.1148/radiology.201.2.8888253
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发表时间：
1996-11-01
期刊：
影响因子：
19.7
通讯作者：
Katsuyama, T
中科院分区：
文献类型：
--
作者：
Seo, GS;Aoki, J;Katsuyama, T
目得：材料和方法：研究了Ⅱ型胶原和硫酸软骨素模型的磁化传递效应（浓度为0.6%、1.2%、2.4%、4.8%、9.6%和19.2%）、大量培养的大鼠骨肉瘤来源的细胞和正常膝关节软骨。胶原蛋白的MT效应产生10%（1.2%胶原蛋白）至31%（19.2%胶原蛋白）的信号强度的降低;该降低随胶原蛋白浓度线性变化。硫酸软骨素没有统计学显著的MT效应。细胞团块显示出22%-26%的信号强度降低，这大于4.8%胶原蛋白的信号强度降低。结论：胶原在透明软骨MT对比中起主要作用。此外，还证明了MT效应的更高细胞贡献。
PURPOSE: To elucidate the magnetization transfer (MT) effects of major componeMATERIALS AND METHODS: The authors evaluated the MT effect of type II collagen and chondroitin sulfate phantoms (concentrations of 0.6%, 1.2%, 2.4%, 4.8%, 9.6%, and 19.2%), mass-cultured rat osteosarcoma-derived cells, and normal articular cartilage of the knee.RESULTS: The MT effect of collagen yielded a decrease in signal intensity of 10% (1.2% collagen) to 31% (19.2% collagen); the decrease varied linearly with collagen concentration. Chondroitin sulfate did not have a statistically significant MT effect. Cell pellets showed a decrease in signal intensity of 22%-26%, which was greater than that of 4.8% collagen. Normal articular cartilage of the knees showed a decrease in signal intensity of 38%-48%.CONCLUSION: The major role of collagen in MT contrast of hyaline cartilage was confirmed. In addition, a higher cellular contribution to the MT effect was also demonstrated.