The C-terminal domain of RNA polymerase II couples mRNA processing to transcription
The C-terminal domain of RNA polymerase II couples mRNA processing to transcription
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DOI：
10.1038/385357a0
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发表时间：
1997-01-23
期刊：
影响因子：
64.8
通讯作者：
Bentley, DL
中科院分区：
文献类型：
--
作者：
McCracken, S;Fong, N;Bentley, DL
信使RNA通过RNA聚合酶II（pol II）转录产生，随后加工初级转录物。转录，剪接和切割聚腺苷酸化可以独立地发生在体外，但我们在这里证明，这些过程是密切相关的体内。我们表明，羧基末端结构域（CTD）的聚合酶II大亚基是需要有效的RNA加工。剪切、3'末端的加工和poly（A）位点下游的转录终止都被CTD的截短抑制。我们发现，裂解-多聚腺苷酸化因子CPSF和CstF特异性地结合到CTD亲和柱上，并在高分子量复合物中与pol II共纯化。我们对CTD和3 '-加工因子之间的关联的证明，以及与剪接因子类似相互作用的报道（1，2），表明存在一个mRNA“工厂”，它进行mRNA前体的偶联转录、剪接和切割-聚腺苷酸化。
Messenger RNA is produced by RNA polymerase II (pol II) transcription, followed by processing of the primary transcript. Transcription, splicing, and cleavage-polyadenylation can occur independently in vitro, but we demonstrate here that these processes are intimately linked in vivo. We show that the carboxy-terminal domain (CTD) of the pol II large subunit is required for efficient RNA processing. Splicing, processing of the 3' end and termination of transcription downstream of the poly(A) site, are all inhibited by truncation of the CTD. We found that the cleavage-polyadenylation factors CPSF and CstF specifically bound to CTD affinity columns and copurified with pol II in a high-molecular-mass complex. Our demonstration of an association between the CTD and 3'-processing factors, considered together with reports of a similar interaction with splicing factors(1,2), suggests that an mRNA 'factory' exists which carries out coupled transcription, splicing and cleavage-polyadenylation of mRNA precursors.