Primary Analysis and Construction of Ethephon-induced Latex SSH cDNA Library from Hevea brasiliensis
Primary Analysis and Construction of Ethephon-induced Latex SSH cDNA Library from Hevea brasiliensis
复制标题
DOI：
--
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Catas Haikou
中科院分区：
文献类型：
--
作者：
Catas Haikou
为阐明乙烯利刺激巴西橡胶树胶乳产量提高的分子机理，以乙烯利刺激12 h的胶乳和未处理的对照胶乳为材料，构建了差异表达cDNA的SSH文库。通过蓝白菌落筛选和PCR技术，共获得118条ESTs，对其中25个阳性克隆进行了测序，通过Genbank中的blastx分析，发现5个EST属于未知序列，6个为功能未知的蛋白质基因，2个为功能未知的蛋白质基因，3个与已知的相应基因高度相似。我们对203号EST的表达谱进行了检测，发现它只在乙烯利诱导的胶乳中有转录，在叶片中没有转录，表达强度与施用乙烯利后的时间有关。
A SSH library of differently expressed cDNA was constructed employing two kinds of latex,latex pro-duced under ethephon stimulation for 12 h,and the control without any treatment,in order to illuminate the molecular mechanism underlying higher latex yield in Hevea brasiliensis stimulated by Eth.118 ESTs were ob-tained using the technique of screening the blue-white colony and PCR,in which the 25 selected positive clones were sequenced,indicating 5 ESTs fall into no-clued unknown sequences,6 for the deduced protein genes with unknown functions,2 for the unknown function protein genes,and 3 have high similarity with the known corre-sponding gene respectively,through blastx analysis in Genbank.We checked the expressing profiles of No 203 EST indicating that its transcripts were detected only in the ethphon-induced latex and no transcripts in the leaves,and the expressing intensity depended on the time after application of ethephon.