Cell subsets responding to purified hepatocytes and evidence of indirect recognition of hepatocyte major histocompatibility complex class I antigen. II. In vitro-generated "memory" cells to class I+ class II- hepatocytes.
Cell subsets responding to purified hepatocytes and evidence of indirect recognition of hepatocyte major histocompatibility complex class I antigen. II. In vitro-generated "memory" cells to class I+ class II- hepatocytes.
复制标题
对纯化肝细胞作出反应的细胞亚群以及间接识别肝细胞主要组织相容性复合物 I 类抗原的证据。
DOI：
10.1097/00007890-199204000-00029
复制
发表时间：
1992
期刊：
影响因子：
6.2
通讯作者：
Matas,AJ
中科院分区：
文献类型：
--
作者：
Bumgardner,GL;Chen,S;Almond,PS;Bach,FH;Ascher,NL;Matas,AJ
纯化的肝细胞在原代混合淋巴细胞肝细胞培养物（MLHC）中5天后刺激幼稚脾细胞产生L3 T4-、Ly 2+同种特异性溶细胞性T细胞。研究表明，纯化肝细胞的免疫原性与MHC I类抗原的表达有关。以下实验的目的是鉴定特异性识别肝细胞MHC I类抗原的细胞亚群。我们采用致敏淋巴细胞试验（PLT），以测试“第二组”的反应。来自原代MLHC的细胞在培养物中再生长7-9天时恢复到功能性静止状态。然后测试细胞的细胞毒性或用同种异体、同基因或“第三方”肝细胞再激发并测试增殖。MLHC在第12天的同种细胞毒性较低，但致敏细胞群在再刺激后48小时对同种异体肝细胞的反应中表现出峰值增殖。当大量PLT细胞免疫耗竭时，L3 T4+，Ly 2-和L3 T4-，Ly 2 + T细胞亚群均表现出对同种异体肝细胞的“第二组”应答，这与对肝细胞MHC I类同种异体抗原的特异性识别和“记忆”保留一致。
Purified hepatocytes stimulate the development of L3T4-, Ly2+ allospecific cytolytic T cells from naive splenocytes after 5 days in primary mixed lymphocytehepatocyte culture (MLHC). Previous studies indicate that the immunogenicity of purified hepatocytes relates to the expression of MHC class I antigen. The purpose of the following experiments was to identify the cell subsets that specifically recognize hepatocyte MHC class I antigen. We employed primed lymphocyte testing (PLT) in order to test for a “second set” response. Cells from primary MLHC reverted to a functionally quiescent state when they were grown in culture for an additional 7–9 days. The cells were then tested for cytotoxicity or rechallenged with allogeneic, syngeneic, or “third party” hepatocytes and tested for proliferation. Allocytotoxicity was low on day 12 in MLHC, but the sensitized cell population demonstrated peak proliferation in response to allogeneic hepatocytes 48 hr after restimulation. When bulk PLT cells were immunodepleted, both L3T4+, Ly2-and L3T4-, Ly2+ T cell subsets demonstrated a “second set” response to allogeneic hepatocytes consistent with specific recognition of and retention of “memory” for hepatocyte MHC class I alloantigen.
对纯化肝细胞作出反应的细胞亚群以及间接识别肝细胞主要组织相容性复合物 I 类抗原的证据。
影响因子：
6.2
作者：
G. Bumgardner;Sally C. Chen;P. Almond;Fritz H. Bach;N. Ascher;A. J. Matas
通讯作者：
A. J. Matas