Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry: Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes
Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry: Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes
复制标题
DOI：
10.1021/acs.jafc.6b05278
复制
发表时间：
2017-08-09
影响因子：
6.1
通讯作者：
Teresa Escribano-Bailon, M.
中科院分区：
文献类型：
--
作者：
Garcia-Estevez, Ignacio;Alcalde-Eon, Cristina;Teresa Escribano-Bailon, M.
由于单体、低聚物和聚合物的结构相似，食品基质中黄烷醇组成的详细测定不是一项简单的任务。本研究的目的是建立和验证HPLC-MS/ ms -多重反应监测（MRM）方法，该方法可以准确和精确地定量儿茶素、没食子儿茶素和低聚原花青素。该方法具有较高的相关系数（>0.993），加样回收率与100%无统计学差异，日内、日间精密度(
The determination of the detailed flavanol composition in food matrices is not a simple task because of the structural similarities of monomers and, consequently, oligomers and polymers. The aim of this study was the development and validation of an HPLC-MS/MS-multiple reaction monitoring (MRM) method that would allow the accurate and precise quantification olcatechins, gallocatechins, and oligomeric proanthocyanidins. The high correlation coefficients of the calibration curies (>0.993), the recoveries not statistically different from 100%, the good intra- and interday precisions (