Isolation and characterization of genes encoding polycyclic aromatic hydrocarbon dioxygenase from acenaphthene and acenaphthylene degrading Sphingomonas sp. strain A4.
Isolation and characterization of genes encoding polycyclic aromatic hydrocarbon dioxygenase from acenaphthene and acenaphthylene degrading Sphingomonas sp. strain A4.
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DOI：
10.1111/j.1574-6968.2004.tb09770.x
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发表时间：
2004-09
影响因子：
2.1
通讯作者：
O. Pinyakong;H. Habe;A. Kouzuma;H. Nojiri;H. Yamane;T. Omori
中科院分区：
文献类型：
--
作者：
O. Pinyakong;H. Habe;A. Kouzuma;H. Nojiri;H. Yamane;T. Omori
Sphingomonas sp.菌株A4能够利用汽油和汽油作为唯一的碳源和能源，但不能生长在其他多环芳烃(PAHs)上。从该菌株中分离到编码环羟化双加氧酶末端加氧酶组分(arhA1和arhA2)的基因，该克隆含有菲降解Sphingobium sp的电子传递蛋白。菌株P2。ArhA1和arhA2的翻译产物与其他环羟化双加氧酶的大小亚基的序列具有中等的同源性(小于56%)。重组大肠杆菌克隆的生物转化结果表明，该加氧酶对几种多环芳烃具有广泛的底物专一性，这些多环芳烃包括菲、菲、菲和荧菲。Southern杂交分析表明，在与arhA1基因不同的基因座上存在一个可能的arhA1同源物。在菌株A4中插入失活的arhA1基因表明，该基因而不是推测的同源基因参与了该菌株对汽油和汽油的降解。
Sphingomonas sp. strain A4 is capable of utilizing acenaphthene and acenaphthylene as sole carbon and energy sources, but it is unable to grow on other polycyclic aromatic hydrocarbons (PAHs). The genes encoding terminal oxygenase components of ring-hydroxylating dioxygenase (arhA1 and arhA2) were isolated from this strain by means of the ability to oxidize indole to indigo of the Escherichia coli clone containing electron transport proteins from phenanthrene-degrading Sphingobium sp. strain P2. The translated products of arhA1 and arhA2 exhibited moderate sequence identity (less than 56%) to large and small subunits of dioxygenase of other ring-hydroxylating dioxygenases. Biotransformation with recombinant E. coli clone revealed the broad substrate specificity of this oxygenase toward several PAHs including acenaphthene, acenaphthylene, naphthalene, phenanthrene, anthracene and fluoranthene. Southern hybridization analysis revealed the presence of a putative arhA1 homologue on a locus different from that of the arhA1 gene. Insertion inactivation of the arhA1 gene in strain A4 suggested that the gene but not the putative homologue one was involved in the degradation of acenaphthene and acenaphthylene in this strain.