Mitochondrial and cytosolic ATP/ADP ratios in isolated hepatocytes. A comparison of the digitonin method and the non-aqueous fractionation procedure.
Mitochondrial and cytosolic ATP/ADP ratios in isolated hepatocytes. A comparison of the digitonin method and the non-aqueous fractionation procedure.
复制标题
离体肝细胞中线粒体和胞质 ATP/ADP 比率。
作者：
S. Soboll;T. Akerboom;W. D. Schwenke;R. Haase;H. Sies
用洋地黄素和非水提法分离的6种不同制备的肝细胞，线粒体基质中的ATP含量/ADP含量的比值分别为2.07+/-0.21和2.26+/-0.22。相反，采用非水相分离的无血红蛋白灌流肝脏测定的线粒体基质ATP/ADP约为0.2，而用分离的细胞和完整的器官测得的胞浆值相似。结论是，分离的肝细胞线粒体基质中相对较高的ATP/ADP比率反映了由于模型的性质而不是方法学上的人工制品而导致的生化差异。
The ratio of ATP content/ADP content in the mitochondrial matrix was found to be 2.07 +/- 0.21 and 2.26 +/- 0.22 as determined with six different preparations of isolated hepatocytes subfractionated with the digitonin and non-aqueous-fractionation procedures, respectively. In contrast, the mitochondrial matrix ATP/ADP determined with isolated haemoglobin-free perfused liver by using the non-aqueous-fractionation procedure was about 0.2, whereas the cytosolic values obtained with isolated cells and with the intact organ were similar. It is concluded that the relatively higher ATP/ADP ratio in the mitochondrial matrix of isolated hepatocytes represents a biochemical difference due to properties of the model rather than a methodological artifact.