Rapid increase in pertactin-deficient Bordetella pertussis isolates, Australia.
Rapid increase in pertactin-deficient Bordetella pertussis isolates, Australia.
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DOI：
10.3201/eid2004.131478
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发表时间：
2014-04
影响因子：
11.8
通讯作者：
Lan R
中科院分区：
文献类型：
--
作者：
Lam C;Octavia S;Ricafort L;Sintchenko V;Gilbert GL;Wood N;McIntyre P;Marshall H;Guiso N;Keil AD;Lawrence A;Robson J;Hogg G;Lan R
澳大利亚于1997年推出了百日咳杆菌无细胞疫苗。到2000年，这些疫苗已经取代了全细胞疫苗。2008-2012年期间，发生了一次百日咳大爆发。在此期间，30%(96/320)的百日咳杆菌分离株不表达疫苗抗原pertactin(Prn)。Prn失活的机制有多种，包括IS481和IS1002中断、同源多聚体中的变异和prn基因的缺失。在序列水平上无法确定16株(17%)分离株缺乏prn表达的机制。这些发现表明，自2008年以来，不表达prn的百日咳杆菌独立出现了多次，而不是通过扩增单个prn阴性克隆。除1株外，所有分离株都有ptxA1、PRN2和ptxP3等位基因，这些等位基因代表了目前在澳大利亚流行的菌株。这种模式与百日咳杆菌在疫苗选择压力下的持续进化是一致的。
Acellular vaccines against Bordetella pertussis were introduced in Australia in 1997. By 2000, these vaccines had replaced whole-cell vaccines. During 2008–2012, a large outbreak of pertussis occurred. During this period, 30% (96/320) of B. pertussis isolates did not express the vaccine antigen pertactin (prn). Multiple mechanisms of prn inactivation were documented, including IS481 and IS1002 disruptions, a variation within a homopolymeric tract, and deletion of the prn gene. The mechanism of lack of expression of prn in 16 (17%) isolates could not be determined at the sequence level. These findings suggest that B. pertussis not expressing prn arose independently multiple times since 2008, rather than by expansion of a single prn-negative clone. All but 1 isolate had ptxA1, prn2, and ptxP3, the alleles representative of currently circulating strains in Australia. This pattern is consistent with continuing evolution of B. pertussis in response to vaccine selection pressure.