Genome-wide identification and expression analysis of the fatty acid desaturase genes in Medicago truncatula
Genome-wide identification and expression analysis of the fatty acid desaturase genes in Medicago truncatula
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蒺藜苜蓿脂肪酸去饱和酶基因的全基因组鉴定及表达分析
DOI：
10.1016/j.bbrc.2018.03.165
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发表时间：
2018
影响因子：
3.1
通讯作者：
Wang Yanrong
中科院分区：
文献类型：
--
作者：
Zhang Zhengshe;Wei Xingyi;Liu Wenxian;Min Xueyang;Jin Xiaoyu;Ndayambaza Boniface;Wang Yanrong
脂肪酸去饱和酶（FADs）是一类重要的脂肪酸酶，在调节植物脂肪酸组成中起着重要作用。本研究通过数据库检索，在蒺藜苜蓿中鉴定出20个全长FAD基因。FAD基因在除第6、8号染色体外的6条染色体上分布不均匀。系统发育分析表明，蒺藜苜蓿FAD基因可聚为6个亚家族，且同一亚家族中FAD基因的外显子数目和内含子相位相似。此外，基于qRT-PCR的表达分析表明，FAD基因广泛参与冷、热反应。本研究为进一步克隆蒺藜苜蓿及其它豆科植物FAD基因及其功能研究奠定了基础。
Fatty acid desaturases (FADs) are of great importance and play critical roles in regulating plant fatty acid (FA) compositions. But to date, no reports about characterization of theFADgenes have been reported in the model dicotyledonous grass speciesMedicagotruncatula.In this study, using database searches, 20 full-lengthFADgenes were identified inM. truncatula. TheseFADgenes were unevenly distributed on six chromosomes except the chromosome 6 and 8. Phylogenetic analysis showed theFADgenes inM. truncatulawere clustered into six subfamilies and had similar exon number and intron phase in the same subfamily. Moreover, expression analysis based on qRT-PCR indicated theseFADgenes were extensively involved in cold and heat responses. This study would provide an important foundation for future cloning and functional studies ofFADgenes inM. truncatulaand other related legume species.