A new method of fabricating a blend scaffold using an indirect three-dimensional printing technique
A new method of fabricating a blend scaffold using an indirect three-dimensional printing technique
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DOI：
10.1088/1758-5090/7/4/045003
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发表时间：
2015-12-01
期刊：
影响因子：
9
通讯作者：
Cho, Dong-Woo
中科院分区：
文献类型：
--
作者：
Jung, Jin Woo;Lee, Hyungseok;Cho, Dong-Woo
由于其简单性和有效性，聚合物的物理共混被认为是开发具有所需机械和生物化学性质的通用支架的实用策略。在目前的工作中，提出了一种间接三维（i3 D）打印技术，使用不混溶材料的混合物，如聚己内酯（PCL）和明胶制造3D自由形式的支架。i3 D打印技术包括模具的3D打印和牺牲成型工艺。将PCL/氯仿和明胶/水物理混合以制备共混溶液，随后将其注入3D打印模具的腔体中。在去除溶剂和明胶交联后，将模具溶解以获得具有特定三维结构的PCL-明胶（PG）支架。扫描电子显微镜和傅里叶变换红外光谱分析表明，聚己内酯质量和明胶纤维在PG支架均匀共存，没有化学键合。压缩试验证实，明胶掺入PCL增强了其机械柔韧性和柔软性，适合于软组织工程的点，而不是纯PCL。与PCL支架相比，PG支架上培养的人脂肪源性干细胞表现出增强的体外软骨分化和组织形成。i3 D打印技术可用于混合各种材料，促进特定组织再生的3D支架制造。此外，这种方便和通用的技术可能会导致3D打印在组织工程中的更广泛应用。
Due to its simplicity and effectiveness, the physical blending of polymers is considered to be a practical strategy for developing a versatile scaffold having desirable mechanical and biochemical properties. In the present work, an indirect three-dimensional (i3D) printing technique was proposed to fabricate a 3D free-form scaffold using a blend of immiscible materials, such as polycaprolactone (PCL) and gelatin. The i3D printing technique includes 3D printing of a mold and a sacrificial molding process. PCL/chloroform and gelatin/water were physically mixed to prepare the blend solution, which was subsequently injected into the cavity of a 3D printed mold. After solvent removal and gelatin cross-linking, the mold was dissolved to obtain a PCL-gelatin (PG) scaffold, with a specific 3Dstructure. Scanning electron microscopy and Fourier transform infrared spectroscopy analysis indicated that PCL masses and gelatin fibers in the PG scaffold homogenously coexisted without chemical bonding. Compression tests confirmed that gelatin incorporation into the PCL enhanced its mechanical flexibility and softness, to the point of being suitable for soft-tissue engineering, as opposed to pure PCL. Human adipose-derived stem cells, cultured on a PG scaffold, exhibited enhanced in vitro chondrogenic differentiation and tissue formation, compared with those on a PCL scaffold. The i3D printing technique can be used to blend a variety of materials, facilitating 3D scaffold fabrication for specific tissue regeneration. Furthermore, this convenient and versatile technique may lead to wider application of 3D printing in tissue engineering.