Molecular cloning, characterization and expression analysis of the fatty acid-binding protein (MnFABP), involved in dietary lipid sources response in oriental river prawn, Macrobrachium nipponense
Molecular cloning, characterization and expression analysis of the fatty acid-binding protein (MnFABP), involved in dietary lipid sources response in oriental river prawn, Macrobrachium nipponense
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DOI：
10.1111/anu.12088
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发表时间：
2014-08-01
影响因子：
3.5
通讯作者：
Kong, Y. Q.
中科院分区：
文献类型：
--
作者：
Ding, Z. L.;Chen, L. Q.;Kong, Y. Q.
从日本沼虾（Macrobrachium nipponense）中克隆了一个脂肪酸结合蛋白（FABP）基因，命名为MnFABP 10。MnFABP 10的cDNA全长为646 bp，编码130个氨基酸。实时荧光定量RT-PCR结果表明，MnFABP 10基因在不同组织中均有表达，其中在肝胰腺中的表达量最高。MnFABP 10 mRNA在肝胰腺和卵巢中的表达水平均高于对照组。不同发育阶段的卵巢发育差异显著。Western blot结果显示，一个单一的免疫反应带，估计分子量约为14 kDa的发育卵巢。然后，M。用牛油（BT）、大豆油（SO）、青鳕油（FO）和鱼油与大豆油的混合物（FO/SO 2：1 w/w）4种等氮等热量日粮饲喂初始体重为0.090 ± 0.0010 g的日本对虾（Nipponense）52 d。肝胰腺中MnFABP 10的mRNA水平受不同脂质来源的影响，在SO组中出现表达高峰。本研究提示MnFABP 10可能在卵巢成熟过程中起一定作用，其mRNA在肝胰腺中的表达受饲料中脂类来源的调控。日本的。
A fatty acid-binding protein (FABP) gene designated as MnFABP10 was cloned and characterized from the freshwater prawn Macrobrachium nipponense. The full-length cDNA of MnFABP10 was 646 bp encoding a 130 amino acid. Real-time quantitative RT-PCR showed that the MnFABP10 gene was expressed in various tissues with the highest expression in the hepatopancreas. The MnFABP10 mRNA levels in the hepatopancreas and ovary of M. nipponense were dependent on the stages of ovarian development. Western blot results revealed a single immunoreactive band with an estimated molecular mass of approximate 14 kDa in the developmental ovary. Then, M. nipponense with an initial body weight of 0.090 +/- 0.0010 g were fed with four isonitrogenous and isocaloric diets with different oils, that is, beef tallow (BT), soybean oil (SO), pollack fish oil (FO) and a mixture of fish oil and soybean oil (FO/SO 2 : 1 w/w) for 52 days. The mRNA levels of MnFABP10 in the hepatopancreas were influenced by different lipid sources, with a peak expression observed in prawns fed SO. This study suggests that MnFABP10 may have a putative function in ovary maturation, and its mRNA expression in the hepatopancreas can be regulated by the source of dietary lipids in M. nipponense.