Identification of a novel antiapoptotic protein, GAM-1, encoded by the CELO adenovirus
Identification of a novel antiapoptotic protein, GAM-1, encoded by the CELO adenovirus
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DOI：
10.1128/jvi.71.4.3168-3177.1997
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发表时间：
1997-04-01
影响因子：
5.4
通讯作者：
Cotten, M
中科院分区：
文献类型：
--
作者：
Chiocca, S;Baker, A;Cotten, M
我们已经开发了一种简单的筛选方法来鉴定基因，以模拟bcl-2或腺病毒E1B 19K在转染后提高细胞存活率，并使用这种方法在禽腺病毒CELO中鉴定了这样的基因。该基因编码一种新的30 kda核蛋白，我们将其命名为GAM-1，其在阻断细胞凋亡方面的功能与Bcl-2和腺病毒E1B 19K相当。然而，GAM-1与Bcl-2、E1B 19K或任何其他已知的抗凋亡蛋白没有序列同源性，因此定义了一种新的抗凋亡功能。
We have developed a simple screening method to identify genes that mimic bcl-2 or adenovirus E1B 19K in enhancing cell survival after transfection and have used this method to identify such a gene in the avian adenovirus CELO. The gene encodes a novel 30-kDa nuclear protein, which we have named GAM-1, that functions comparably to Bcl-2 and adenovirus E1B 19K in blocking apoptosis. However, GAM-1 has no sequence homology to Bcl-2, E1B 19K, or any other known antiapoptotic proteins and thus defines a novel antiapoptotic function.