Liquid-chromatographic analysis for urinary porphyrins.
Liquid-chromatographic analysis for urinary porphyrins.
复制标题
尿卟啉的液相色谱分析。
作者：
R. E. Ford;C. Ou;R. Ellefson
在由μ-Bondapak C18固定相和甲醇和磷酸钠水溶液（pH 3.5）的线性梯度移动的相组成的系统中，根据羧基的数量分离尿卟啉。通过将5 mL样品的pH值调节至2.0并通过离心去除固体，简单地制备样品。用荧光法测量洗脱的卟啉。天然存在的非卟啉荧光物质在卟啉之前洗脱。色谱法需要约20分钟，重新建立初始条件需要额外的15分钟。
Urinary porphyrins are separated, according to number of carboxyl groups, in a system consisting of a mu-Bondapak C18 stationary phase and a mobile phase of methanol and aqueous sodium phosphate (pH 3.5) in a linear gradient. The specimen is prepared simply by adjusting the pH of a 5-mL sample to 2.0 and removing solids by centrifugation. The eluted porphyrins are measured fluorometrically. Naturally occurring non-porphyrin fluorescent substances are eluted ahead of the porphyrins. Chromatography requires about 20 min, and a re-establishment of initial conditions requires an additional 15 min.